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Betto, Riccardo Massimiliano (2018) Role of mitochondrial STAT3 in the metabolism of mouse embryonic stem cells. [Ph.D. thesis]

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Abstract (english)

Stat3 is a transcription factor activated by the cytokine LIF (leukemia inhibitory factor) in mouse embryonic stem (ES) cells. The LIF/Stat3 axis maintains ES cell self-renewal through the induction of nuclear target genes such as Klf4 and Tfcp2l1. Here I report that Stat3 has a parallel function as a regulator of mitochondrial activity. Stat3 binds the mitochondrial DNA and increases the transcription of subunits of the respiratory chain, leading to increased respiration and optimal ES cell proliferation. Indeed, deletion of Stat3 results in reduced respiration and proliferation of ES cells.

Abstract (italian)

Stat3 è un fattore di trascrizione attivato dalla citochina LIF (leukemia inhibitory factor) nelle cellule staminali embrionali murine. La via di segnale di LIF/ Stat3 è in grado di mantenere il self-renewal e la pluripotenza attraverso l’induzione di target nucleari come Klf4 e Tfcp2l1. In questa tesi di Dottorato è riportato che Stat3, in aggiunta alla propria funzione nucleare, regola anche l’attività mitocondriale. Stat3 è in grado di legare il DNA mitocondriale e incrementare la trascrizione delle subunità della catena respiratoria, permettendo l’aumento della respirazione con un conseguente aumento della proliferazione delle cellule embrionali staminali murine. Infatti la delezione del gene Stat3 in queste cellule causa una riduzione sia della respirazione che della proliferazione.

EPrint type:Ph.D. thesis
Tutor:Martello , Graziano
Ph.D. course:Ciclo 30 > Corsi 30 > MEDICINA MOLECOLARE
Data di deposito della tesi:13 January 2018
Anno di Pubblicazione:13 January 2018
Key Words:Key words: LIF/Stat3 Embryonic stem cells Mitochondria Metabolism
Settori scientifico-disciplinari MIUR:Area 05 - Scienze biologiche > BIO/11 Biologia molecolare
Struttura di riferimento:Dipartimenti > Dipartimento di Medicina Molecolare
Codice ID:10776
Depositato il:09 Nov 2018 09:41
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