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Ferrari, Francesco and Bortoluzzi, Stefania and Coppe, Alessandro and Sirota, Alexandra and Safran, Marilyn and Shmoish, Michael and Ferrari, Sergio and Lancet, Doron and Danieli, Gian Antonio and Bicciato, Silvio (2007) Novel definition files for human GeneChips based on GeneAnnot. [Online journal papers]

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Per gentile concessione di: http://www.biomedcentral.com/1471-2105/8/446

Abstract (english)

Background

Improvements in genome sequence annotation revealed discrepancies in the original probeset/gene assignment in Affymetrix microarray and the existence of differences between annotations and effective alignments of probes and transcription products. In the current generation of Affymetrix human GeneChips, most probesets include probes matching transcripts from more than one gene and probes which do not match any transcribed sequence.

Results

We developed a novel set of custom Chip Definition Files (CDF) and the corresponding Bioconductor libraries for Affymetrix human GeneChips, based on the information contained in the GeneAnnot database. GeneAnnot-based CDFs are composed of unique custom-probesets, including only probes matching a single gene.

Conclusion

GeneAnnot-based custom CDFs solve the problem of a reliable reconstruction of expression levels and eliminate the existence of more than one probeset per gene, which often leads to discordant expression signals for the same transcript when gene differential expression is the focus of the analysis. GeneAnnot CDFs are freely distributed and fully compliant with Affymetrix standards and all available software for gene expression analysis. The CDF libraries are available from http://www.xlab.unimo.it/GA_CDF webcite, along with supplementary information (CDF libraries, installation guidelines and R code, CDF statistics, and analysis results).


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EPrint type:Online journal papers
Anno di Pubblicazione:2007
Key Words:GeneChips, genome
Settori scientifico-disciplinari MIUR:Area 06 - Scienze mediche > MED/03 Genetica medica
Struttura di riferimento:Dipartimenti > Dipartimento di Biologia
Dipartimenti > pre 2012 - Dipartimento di Processi chimici dell'Ingegneria
Codice ID:1185
Depositato il:09 Dec 2008
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