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Di Lisi, Raffaella and Picard, Anne and Ausoni, Simonetta and Schiaffino, Stefano (2007) GATA elements control repression of cardiac troponin I promoter activity in skeletal muscle cells. [Online journal papers]

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Per gentile concessione di: http://www.biomedcentral.com/1471-2199/8/78

Abstract (english)

Background

We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter GATA elements in skeletal muscle cells.

Results

Mutation or deletion of GATA elements induces a strong transcriptional activation of the cTnI promoter in regenerating skeletal muscle and in cultured skeletal muscle cells. Electrophoretic mobility shift assays show that proteins present in nuclear extracts of C2C12 muscle cells bind the GATA motifs present in the cTnI promoter. However, GATA protein complex formation is neither reduced nor supershifted by antibodies specific for GATA-2, -3 and -4, the only GATA transcripts present in muscle cells.

Conclusion

These findings indicate that the cTnI gene promoter is repressed in skeletal muscle cells by GATA-like factors and open the way to further studies aimed at identifying these factors.


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EPrint type:Online journal papers
Anno di Pubblicazione:2007
Key Words:cardiac-specific, genes, cardiac muscle
Settori scientifico-disciplinari MIUR:Area 06 - Scienze mediche > MED/26 Neurologia
Struttura di riferimento:Dipartimenti > pre 2012 - Dipartimento di Scienze Biomediche Sperimentali
Codice ID:1201
Depositato il:09 Dec 2008
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