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Sommer, Ingolf - Toppo, Stefano - Sander, Olivier - Lengauer, Thomas - Tosatto, Silvio CE (2006) Improving the quality of protein structure models by selecting from alignment alternatives. [Articolo di periodico (online)]

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Per gentile concessione di: http://www.biomedcentral.com/1471-2105/7/364

Abstract (inglese)

Background

In the area of protein structure prediction, recently a lot of effort has gone into the development of Model Quality Assessment Programs (MQAPs). MQAPs distinguish high quality protein structure models from inferior models. Here, we propose a new method to use an MQAP to improve the quality of models. With a given target sequence and template structure, we construct a number of different alignments and corresponding models for the sequence. The quality of these models is scored with an MQAP and used to choose the most promising model. An SVM-based selection scheme is suggested for combining MQAP partial potentials, in order to optimize for improved model selection.

Results

The approach has been tested on a representative set of proteins. The ability of the method to improve models was validated by comparing the MQAP-selected structures to the native structures with the model quality evaluation program TM-score. Using the SVM-based model selection, a significant increase in model quality is obtained (as shown with a Wilcoxon signed rank test yielding p-values below 10-15). The average increase in TMscore is 0.016, the maximum observed increase in TM-score is 0.29.

Conclusion

In template-based protein structure prediction alignment is known to be a bottleneck limiting the overall model quality. Here we show that a combination of systematic alignment variation and modern model scoring functions can significantly improve the quality of alignment-based models.


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Tipo di EPrint:Articolo di periodico (online)
Anno di Pubblicazione:2006
Parole chiave (italiano / inglese):protein structure prediction, alignment alternatives
Settori scientifico-disciplinari MIUR:Area 05 - Scienze biologiche > BIO/11 Biologia molecolare
Struttura di riferimento:Dipartimenti > Dipartimento di Chimica Biologica
Dipartimenti > Dipartimento di Biologia
Codice ID:1214
Depositato il:09 Dic 2008
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