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Tiozzo, Caterina (2009) Role of Pten in lung development. [Ph.D. thesis]

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Abstract (english)

Rationale: Pten is a tumor-suppressor gene, involved in stem cell homeostasis and tumorigenesis. In mouse, Pten expression is ubiquitous and begins as early as 7 days of gestation. Pten-/- mouse embryos die early during gestation indicating a critical role for Pten in embryonic development.
Objective: To test the role of Pten in lung development and injury, we conditionally deleted Pten throughout the lung epithelium by crossing Ptenflox/flox with Nkx2.1-cre driver mice and throughout the lung mesenchyme by crossing Ptenflox/flox with Nkx2.1-cre driver or Dermo1-cre driver. The resulting PtenNkx2.1-cre mutants were analyzed for lung defects and response to injury.
Results: PtenNkx2.1-cre embryonic lungs showed airway epithelial hyperplasia with no branching abnormalities. In vitro culture of mutant lungs also showed an altered responsed to TGF-? when in vivo In adult mice, PtenNkx2.1-cre lungs exhibit increased progenitor cell pools comprised of basal cells in the trachea, CGRP/CC10 double-positive neuroendocrine cells in the bronchi and CC10/SpC double positive cells in the bronchioalveolar duct junction (BADJ). Pten deletion impacted differentiation of various lung epithelial cell lineages, with decreased number of terminally differentiated cells. Over time, PtenNxk2.1-cre epithelial cells residing in the BADJ underwent proliferation, and formed uniform masses, supporting the concept that the cells residing in this distal niche may also be the source of pro-carcinogenic “stem” cells. Finally, increased progenitor cells in all the lung compartments conferred an overall selective advantage to naphthalene injury compared to wild type control mice.
PtenDermo1cre embryonic lungs, moreover, showed normal lung development but increased collagen1 and extracellular matrix production.
Conclusions: Pten has a pivotal role in lung stem cell homeostasis, cell differentiation and consequently resistance to lung injury in the epithelium, Further studies are necessary to clarify the real role of Pten in lung mesenchyme.

Abstract (italian)

Rationale: Pten e’ un gene coinvolto nell’omeostasi delle cellule staminali e nella formazione di tumori. Nei topi, Pten inizia ad essere presente 7 giorni dopo il concepimento. Pten ha un ruolo critico nello sviluppo embrionale: gli embrioni di topo Pten-/-, infatti, muoiono molto presto durante la gestazione.
Scopo dello studio: Studiare il ruolo di Pten nello sviluppo polmonare, eliminando Pten nell’epitelio polmonare, incrociando Ptenflox/flox con topi portatori di Nkx2.1-cre; Pten e’ stato anche eliminato dal mesenchima polmonare incrociando Ptenflox/flox con topi Dermo1-cre. I risultanti PtenNkx2.1cre sono stati analizzati alla ricerca di difetti nello sviluppo polmonare.
Risultati: I polmoni PtenNkx2.1-cre hanno evidenziato in vitro una alterata risposta al TGF-?. In vivo non presentavano nessuna alterazione nel branching bensi una iperplasia polmonare nelle vie aerre. Nei topi adulti, I polmoni PtenNkx2.1-cre presentavano un aumentato pool di cellule progenitori in tutti i distretti: nella trachea, le cellule basali, nei bronchi le cellule neuroepiteliali, positive per CGRP/CC10 ed infine, nella giunzione tra gli alveoli e I bronchi terminali (BADJ), le cellule positive per Spc/CC10. L’assenza di Pten ha un impatto nella differenziazione cellulare, con un diminuito aumento delle cellule all’ultimo stadio di differenziazione. Nel tempo, le cellule epiteliali PtenNxk2.1-cre residenti a livello del BADJ proliferano e formano delle masse di tipo tumorale; questi dati supportano l’idea che le cellule presenti in questa niche possano essere l’origine delle cosidette “pro-carcinogenic stem cells”. L’aumento delle cellule progenitrici, inoltre, conferisce un selettivo vantaggio dopo danno polmonare. I topi con Pten eliminato nell’epitelio, invece, non evidenziavano ne uno sviluppo polmonare alterato ne una alterata differenziazione delle cellule mesenchimali; tuttavia, dimostravano un aumentata deposizione di collagene1 e di matrice extracellulare. Conclusioni: Pten ha un ruolo importante nell’omeostasi delle cellule progenitori del polmone, nella differenziazione epiteliale polmonare e nella resistenza dopo danno. Ulteriori studi sono necessary per chiarire l’esatto ruolo di Pten nel mesenchima polmonare.

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EPrint type:Ph.D. thesis
Tutor:Chiandetti, Lino
Supervisor:MInoo, Parviz and Bellusci, Saverio
Ph.D. course:Ciclo 21 > Scuole per il 21simo ciclo > MEDICINA DELLO SVILUPPO E SCIENZE DELLA PROGRAMMAZIONE > GENETICA BIOCHIMICA E MOLECOLARE
Data di deposito della tesi:28 January 2009
Anno di Pubblicazione:28 January 2009
Key Words:Pten, lung development, lung progenitor cells.
Settori scientifico-disciplinari MIUR:Area 05 - Scienze biologiche > BIO/11 Biologia molecolare
Struttura di riferimento:Dipartimenti > pre 2012 - Dipartimento di Pediatria
Codice ID:1591
Depositato il:28 Jan 2009
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Le url contenute in alcuni riferimenti sono raggiungibili cliccando sul link alla fine della citazione (Vai!) e tramite Google (Ricerca con Google). Il risultato dipende dalla formattazione della citazione.

1. Ali I.U., L.M. Schriml and M. Dean, Mutational spectra of PTEN/MMAC1 gene: a tumor suppressor with lipid phosphatase activity, J. Natl. Cancer Inst. 91 (1999), pp. 1922–1932. Cerca con Google

2. Alison M. R., Vig, P., Russo, F., Bigger, B. W., Amofah, E., Themis, M. and Forbes, S. (2004). Hepatic stem cells: from inside and outside the liver? Cell Prolif. 37, 1-21. Cerca con Google

3. Anderson K.E., J. Coadwell, L.R. Stephens and P.T. Hawkins, Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B, Curr. Biol. 8 (1998), pp. 684–691. Cerca con Google

4. Arch E.M., B.K. Goodman, R.A. Van Wesep, D. Liaw, K. Clarke, R. Parsons, V.A. McKusick, M.T. Geraghty, D.J. Marsh, P.L. Dahia, Z. Zheng, R.J. Gorlin, C. Eng, J. Li, S.I. Wang, S. Bose, K.M. Call, H.C. Tsou and M. Peacocke, Deletion of PTEN in a patient with Bannayan–Riley–Ruvalcaba syndrome suggests allelism with Cowden disease, Am. J. Med. Genet. 71 (1997), pp. 489–493. Cerca con Google

5. Aso, Y., Yoneda, K. and Kikkawa, Y. (1976). Morphologic and biochemical study of pulmonary changes induced by bleomycin in mice. Lab. Invest. 35,558 -568. Cerca con Google

6. Aveyard J.S., A. Skilleter, T. Habuchi and M.A. Knowles, Somatic mutation of PTEN in bladder carcinoma, Br. J. Cancer 80 (1999), pp. 904–908. Cerca con Google

7. Avril-Delplanque, A., Casal, I., Castillon, N., Hinnrasky, J., Puchelle, E. and Peault, B. (2005). Aquaporin-3 expression in human fetal airway epithelial progenitor cells. Stem Cells 23,992 -1001. Cerca con Google

8. Batenburg JJ, Elfring RH. Pre-translational regulation by glucocorticoid of fatty acid and phosphatidylcholine synthesis in type II cells from fetal rat lung. FEBS Lett 307: 164–168, 1992. Cerca con Google

9. Basseres DS, Levantini E, Ji H, Monti S, Elf S, Dayaram T, Fenyus M, Kocher O, Golub T, Wong KK, Halmos B, Tenen DG. Respiratory failure due to differentiation arrest and expansion of alveolar cells following lung-specific loss of the transcription factor C/EBPalpha in mice. Mol Cell Biol 26: 1109–1123, 2006. Cerca con Google

10. Bellusci S. Bellusci, J. Grindley, H. Emoto, N. Itoh and B.L. Hogan, Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung, Development 124 (23) (1997), pp. 4867–4878. Cerca con Google

11. Blenkinsopp, W. K. (1967). Proliferation of respiratory tract epithelium in the rat. Exp. Cell Res. 46,144 -154 Cerca con Google

12. Bonnet D, Dick JE.Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nat Med. 1997 Jul;3(7):730-7. Cerca con Google

13. Bort, R., Martinez-Barbera, J. P., Beddington, R. S. and Zaret, K. S. (2004). Hex homeobox gene-dependent tissue positioning is required for organogenesis of the ventral pancreas. Development 131,797 -806. Cerca con Google

14. Cardone M.H., N. Roy, H.R. Stennicke, G.S. Salvesen, T.F. Franke, E. Stanbridge, S. Frisch and J.C. Reed, Regulation of cell death protease caspase-9 by phosphorylation, Science 282 (1998), pp. 1318–1321. Cerca con Google

15. Cardoso WV, Lü J. Regulation of early lung morphogenesis: questions, facts and controversies. Development. 2006 May;133(9):1611-24. Cerca con Google

16. Cross D.A., D.R. Alessi, P. Cohen, M. Andjelkovich and B.A. Hemmings, Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B, Nature 378 (1995), pp. 785–789. Cerca con Google

17. Dahia P.L., PTEN, a unique tumor suppressor gene, Endocr. Relat. Cancer 7 (2000), pp. 115–129. Cerca con Google

18. Davé V, Childs T, Xu Y, Ikegami M, Besnard V, Maeda Y, Wert SE, Neilson JR, Crabtree GR, Whitsett JA. Calcineurin/Nfat signaling is required for perinatal lung maturation and function. J Clin Invest 116: 2597–2609, 2006. Cerca con Google

19. DeFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, tissue-specific gene expression. J Biol Chem 278: 35574–35583, 2003. Cerca con Google

20. Davé V, Childs T, Whitsett JA. Nuclear factor of activated T cells regulates transcription of the surfactant protein D gene (Sftpd) via direct interaction with thyroid transcription factor-1 in lung epithelial cells. J Biol Chem 279: 34578–34588, 2004. Cerca con Google

21. Davé V, Wert SE, Tanner T, Thitoff AR, Loudy DE, Whitsett JA. Conditional deletion of Pten causes bronchiolar hyperplasia. Am J Respir Cell Mol Biol. 2008 Mar;38(3):337-45. Cerca con Google

22. De Langhe SP, Carraro G, Warburton D, Hajihosseini MK, Bellusci S. Levels of mesenchymal FGFR2 signaling modulate smooth muscle progenitor cell commitment in the lung. Dev Biol. 2006 Nov 1;299(1):52-62. Cerca con Google

23. Del Moral, P. M., Sala, F. G., Tefft, D., Shi, W., Keshet, E., Bellusci, S. and Warburton, D. (2006). VEGF-A signaling through Flk-1 is a critical facilitator of early embryonic lung epithelial to endothelial crosstalk and branching morphogenesis. Dev. Biol. 290,177 -188 Cerca con Google

24. Desmoulière A, Badid C, Bochaton-Piallat ML, Gabbiani G. Apoptosis during wound healing, fibrocontractive diseases and vascular wall injury. Int J Biochem Cell Biol. 1997 Jan;29(1):19-30. Cerca con Google

25. Di Cristofano A., B. Pesce, C. Cordon-Cardo and P.P. Pandolfi, Pten is essential for embryonic development and tumour suppression, Nat. Genet. 19 (1998), pp. 348–355. Cerca con Google

26. Downes C.P., S. Ross, H. Maccario, N. Perera, L. Davidson and N.R. Leslie, Stimulation of PI 3-kinase signaling via inhibition of the tumor suppressor phosphatase, PTEN, Adv Enzyme Regul 47 (2007), pp. 184–194. Cerca con Google

27. Dreher T., H. Zentgraf, U. Abel, A. Kappeler, M.S. Michel, U. Bleyl and R. Grobholz, Reduction of PTEN and p27(kip1) expression correlates with tumor grade in prostate cancer. Analysis in radical prostatectomy specimens and needle biopsies, Virchows Arch. 29 (2004), p. 29. Cerca con Google

28. Ebert M.P., G. Fei, L. Schandl, C. Mawrin, K. Dietzmann, P. Herrera, H. Friess, T.M. Gress and P. Malfertheiner, Reduced PTEN expression in the pancreas overexpressing transforming growth factor-beta 1, Br. J. Cancer 86 (2002), pp. 257–262. Cerca con Google

29. Ehrenkranz RA, Walsh MC, Vohr BR, Jobe AH, Wright LL, Fanaroff AA, Wrage LA,Poole K; National Institutes of Child Health and Human Development Neonatal Research Network.Validation of the National Institutes of Health consensus definition of bronchopulmonary dysplasia. Cerca con Google

Pediatrics. 2005 Dec;116(6):1353-60. Cerca con Google

30. Engelhardt, J. F., Schlossberg, H., Yankaskas, J. R. and Dudus, L. (1995). Progenitor cells of the adult human airway involved in submucosal gland development. Development 121,2031 -2046. Cerca con Google

31. Freeman D.J., A.G. Li, G. Wei, H.H. Li, N. Kertesz, R. Lesche, A.D. Whale, H. Martinez-Diaz, N. Rozengurt, R.D. Cardiff, X. Liu and H. Wu, PTEN tumor suppressor regulates p53 protein levels and activity through phosphatase-dependent and -independent mechanisms, Cancer Cell 3 (2003), pp. 117–130. Cerca con Google

32. Fuchs, E., Tumbar, T. and Guasch, G. (2004). Socializing with the neighbors: stem cells and their niche. Cell 116,769 -778. Cerca con Google

33. Gao X., T.P. Neufeld and D. Pan, Drosophila PTEN regulates cell growth and proliferation through PI3K-dependent and -independent pathways, Dev. Biol. 221 (2000), pp. 404–418. Cerca con Google

34. Gebb, S. A. and Shannon, J. M. (2000). Tissue interactions mediate early events in pulmonary vasculogenesis. Dev. Dyn. 217,159 -169. Cerca con Google

35. Giangreco, A., Reynolds, S. D. and Stripp, B. R. (2002). Terminal bronchioles harbor a unique airway stem cell population that localizes to the bronchoalveolar duct junction. Am. J. Pathol. 161,173 -182. Cerca con Google

36. Gimm O., T. Attie-Bitach, J.A. Lees, M. Vekemans and C. Eng, Expression of the PTEN tumour suppressor protein during human development, Hum. Mol. Genet. 9 (2000), pp. 1633–1639. Cerca con Google

37. Goberdhan and Wilson, 2003 D.C. Goberdhan and C. Wilson, PTEN: tumour suppressor, multifunctional growth regulator and more, Hum. Mol. Genet. 12 (2003), pp. R239–R248 Spec No 2. Cerca con Google

38. Groszer M., R. Erickson, D.D. Scripture-Adams, R. Lesche, A. Trumpp, J.A. Zack, H.I. Kornblum, X. Liu and H. Wu, Negative regulation of neural stem/progenitor cell proliferation by the Pten tumor suppressor gene in vivo, Science 294 (2001), pp. 2186–2189. Cerca con Google

39. He XC, Yin T, Grindley JC, Tian Q, Sato T, Tao WA, Dirisina R, Porter-Westpfahl KS, Hembree M, Johnson T, Wiedemann LM, Barrett TA, Hood L, Wu H, Li L. PTEN-deficient intestinal stem cells initiate intestinal polyposis. Nat Genet. 2007 Feb;39(2):189-98. Cerca con Google

40. Hesse S, Mezger M. Involvement of phenolic metabolites in the irreversible protein-binding of aromatic hydrocarbons: reactive metabolites of [14C]naphthalene and [14C]1-naphthol formed by rat liver microsomes. Mol Pharmacol. 1979 Sep;16(2):667-75. Cerca con Google

41. Hong KU, Reynolds SD, Giangreco A, Hurley CM, Stripp BR. Clara cell secretory protein-expressing cells of the airway neuroepithelial body microenvironment include a label-retaining subset and are critical for epithelial renewal after progenitor cell depletion. Am J Respir Cell Mol Biol. 2001 Jun;24(6):671-81. Cerca con Google

42. H. Huang, C.J. Potter, W. Tao, D.M. Li, W. Brogiolo, E. Hafen, H. Sun and T. Xu, PTEN affects cell size, cell proliferation and apoptosis during Drosophila eye development, Development 126 (1999), pp. 5365–5372. Cerca con Google

43. Huelsken J, Vogel R, Brinkmann V, Erdmann B, Birchmeier C, Birchmeier W. Requirement for beta-catenin in anterior-posterior axis formation in mice. J Cell Biol 148: 567–578, 2000. Cerca con Google

44. Isakson BE, Lubman RL, Seedorf GJ, Boitano S. Modulation of pulmonary alveolar type II cell phenotype and communication by extracellular matrix and KGF. Am J Physiol Cell Physiol. 2001 Oct;281(4):C1291-9. Cerca con Google

45. Ito T, Udaka N, Ikeda M, Yazawa T, Kageyama R, Kitamura H. Significance of proneural basic helix-loop-helix transcription factors in neuroendocrine differentiation of fetal lung epithelial cells and lung carcinoma cells. Histol Histopathol. 2001 Jan;16(1):335-43. Cerca con Google

46. Jelaska A, Strehlow D, Korn JH. Fibroblast heterogeneity in physiological conditions and fibrotic disease. Springer Semin Immunopathol. 1999;21(4):385-95. Cerca con Google

47. Jerina DM, Daly JW, Jeffrey AM, Gibson DT.Cis-1,2-dihydroxy-1,2-dihydronaphthalene: a bacterial metabolite from naphthalene. Arch Biochem Biophys. 1971 Jan;142(1):394-6. Cerca con Google

48. Kai, T. and Spradling, A. (2004). Differentiating germ cells can revert into functional stem cells in Drosophila melanogaster ovaries. Nature 428,564 -569. Cerca con Google

49. Kikuchi A., Regulation of beta-catenin signaling in the Wnt pathway, Biochem. Biophys. Res. Commun. 268 (2000), pp. 243–248. Cerca con Google

50. Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T. Identification of bronchioalveolar stem cells in normal lung and lung cancer, Cell 2005; 121: 823-835. Cerca con Google

51. Kimura, S., Hara, Y., Pineau, T., Fernandez-Salguero, P., Fox, C. H., Ward, J. M. and Gonzalez, F. J. (1996). The T/ebp null mouse: thyroid-specific enhancer-binding protein is essential for the organogenesis of the thyroid, lung, ventral forebrain, and pituitary. Genes Dev. 10,60 -69. Cerca con Google

52. Kimura T., A. Suzuki, Y. Fujita, K. Yomogida, H. Lomeli, N. Asada, M. Ikeuchi, A. Nagy, T.W. Mak and T. Nakano, Conditional loss of PTEN leads to testicular teratoma and enhances embryonic germ cell production, Development 130 (2003), pp. 1691–1700. Cerca con Google

53. Koch R., M. Scholz, M.R. Nelen, K. Schwechheimer, J.T. Epplen and A.G. Harders, Lhermitte–Duclos disease as a component of Cowden's syndrome. Case report and review of the literature, J. Neurosurg. 90 (1999), pp. 776–779. Cerca con Google

54. Lahad JP, Mills GB, Coombes KR. Stem cell-ness: a "magic marker" for cancer. J Clin Invest. 2005 Jun;115(6):1463-7. Cerca con Google

55. Lanza, R. (2006). Essentials of Stem Cell Biology. New York: Academic Press. Cerca con Google

56. Lesche R, Groszer M, Gao J, Wang Y, Messing A, Sun H, Liu X, Wu H. Cre/loxP-mediated inactivation of the murine Pten tumor suppressor gene.Genesis. 2002 Feb;32(2):148-9. Cerca con Google

57. Li D.M. and H. Sun, TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta, Cancer Res. 57 (11) (1997), pp. 2124–2129. Cerca con Google

58. Liaw D., D.J. Marsh, J. Li, P.L. Dahia, S.I. Wang, Z. Zheng, S. Bose, K.M. Call, H.C. Tsou, M. Peacocke, C. Eng and R. Parsons, Germline mutations of the PTEN gene in Cowden disease, an inherited breast and thyroid cancer syndrome, Nat. Genet. 16 (1997), pp. 64–67. Cerca con Google

59. Iida S., Y. Tanaka, H. Fujii, S. Hayashi, M. Kimura, T. Nagareda and K. Moriwaki, A heterozygous frameshift mutation of the PTEN/MMAC1 gene in a patient with Lhermitte–Duclos disease—only the mutated allele was expressed in the cerebellar tumor, Int. J. Mol. Med. 1 (1998), pp. 925–929. Cerca con Google

60. Li D.M. and H. Sun, TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta, Cancer Res. 57 (1997), pp. 2124–2129. Cerca con Google

61. Liaw D., D.J. Marsh, J. Li, P.L. Dahia, S.I. Wang, Z. Zheng, S. Bose, K.M. Call, H.C. Tsou, M. Peacocke, C. Eng and R. Parsons, Germline mutations of the PTEN gene in Cowden disease, an inherited breast and thyroid cancer syndrome, Nat. Genet. 16 (1997), pp. 64–67. Cerca con Google

62. Liliental J., S.Y. Moon, R. Lesche, R. Mamillapalli, D. Li, Y. Zheng, H. Sun and H. Wu, Genetic deletion of the Pten tumor suppressor gene promotes cell motility by activation of Rac1 and Cdc42 GTPases, Curr. Biol. 10 (2000), pp. 401–404. Cerca con Google

63. Liu C, Ikegami M, Stahlman MT, Dey CR, Whitsett JA. Inhibition of alveolarization and altered pulmonary mechanics in mice expressing GATA-6. Am J Physiol Lung Cell Mol Physiol 285: L1246–L1254, 2003. Cerca con Google

64. Liu, J. Y., Nettesheim, P. and Randell, S. H. (1994). Growth and differentiation of tracheal epithelial progenitor cells. Am. J. Physiol. 266,L296 -L307. Cerca con Google

65. Londhe VA, Belperio JA, Keane MP, Burdick MD, Xue YY, Strieter RM.CXCR2/CXCR2 ligand biological axis impairs alveologenesis during dsRNA-induced lung inflammation in mice. Pediatr Res. 2005 Nov;58(5):919-26. Cerca con Google

66. Luukko K., A. Ylikorkala, M. Tiainen and T.P. Makela, Expression of LKB1 and PTEN tumor suppressor genes during mouse embryonic development, Mech. Dev. 83 (1999), pp. 187–190. Cerca con Google

67. Maehama T. and J.E. Dixon, The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate, J. Biol. Chem. 273 (1998), pp. 13375–13378. Cerca con Google

68. Matsumoto, K., Yoshitomi, H., Rossant, J. and Zaret, K. S. (2001). Liver organogenesis promoted by endothelial cells prior to vascular function. Science 294,559 -563. Cerca con Google

69. Maeda Y, Davé V, Whitsett JA.Transcriptional control of lung morphogenesis. Physiol Rev. 2007 Jan;87(1):219-44. Cerca con Google

70. Marino S., P. Krimpenfort, C. Leung, H.A. van der Korput, J. Trapman, I. Camenisch, A. Berns and S. Brandner, PTEN is essential for cell migration but not for fate determination and tumourigenesis in the cerebellum, Development 129 (2002), pp. 3513–3522. Cerca con Google

71. Marsh et al., 1998 D.J. Marsh, P.L. Dahia, S. Caron, J.B. Kum, I.M. Frayling, I.P. Tomlinson, K.S. Hughes, R.A. Eeles, S.V. Hodgson, V.A. Murday, R. Houlston and C. Eng, Germline PTEN mutations in Cowden syndrome-like families, J. Med. Genet. 35 (1998), pp. 881–885. Cerca con Google

72. Minoo, P., Su, G., Drum, H., Bringas, P. and Kimura, S. (1999). Defects in tracheoesophageal and lung morphogenesis in Nkx2.1(-/-) mouse embryos. Dev. Biol. 209, 60-71. Cerca con Google

73. Minoo P.Transcriptional regulation of lung development: emergence of specificity. Respir Res. 2000;1(2):109-15. Cerca con Google

74. Moe-Behrens G.H., F.G. Klinger, W. Eskild, T. Grotmol, T.B. Haugen and M. De Felici, Akt/PTEN signaling mediates estrogen-dependent proliferation of primordial germ cells in vitro, Mol. Endocrinol. 17 (2003), pp. 2630–2638. Cerca con Google

75. Morrisey, E. E., Tang, Z., Sigrist, K., Lu, M. M., Jiang, F., Ip, H. S. and Parmacek, M. S. (1998). GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo. Genes Dev. 12,3579 -3590. Cerca con Google

76. Mucenski ML, Wert SE, Nation JM, Loudy DE, Huelsken J, Birchmeier W, Morrisey EE, Whitsett JA. beta-Catenin is required for specification of proximal/distal cell fate during lung morphogenesis. J Biol Chem 278: 40231–40238, 2003. Cerca con Google

77. Munemitsu S., I. Albert, B. Rubinfeld and P. Polakis, Deletion of an amino-terminal sequence beta-catenin in vivo and promotes hyperphosporylation of the adenomatous polyposis coli tumor suppressor protein, Mol. Cell. Biol. 16 (1996), pp. 4088–4094. Cerca con Google

78. Mutsaers SE, Bishop JE, McGrouther G, Laurent GJ. Mechanisms of tissue repair: from wound healing to fibrosis.Int J Biochem Cell Biol. 1997 Jan;29(1):5-17. Cerca con Google

79. Myers M.P., I. Pass, I.H. Batty, J. Van der Kaay, J.P. Stolarov, B.A. Hemmings, M.H. Wigler, C.P. Downes and N.K. Tonks, The lipid phosphatase activity of PTEN is critical for its tumor suppressor function, Proc. Natl. Acad. Sci. U. S. A. 95 (1998), pp. 13513–13518. Cerca con Google

80. Nelen M.R., W.C. van Staveren, E.A. Peeters, M.B. Hassel, R.J. Gorlin, H. Hamm, C.F. Lindboe, J.P. Fryns, R.H. Sijmons, D.G. Woods, E.C. Mariman, G.W. Padberg and H. Kremer, Germline mutations in the PTEN/MMAC1 gene in patients with Cowden disease, Hum. Mol. Genet. 6 (1997), pp. 1383–1387. Cerca con Google

81. Neshat M.S., I.K. Mellinghoff, C. Tran, B. Stiles, G. Thomas, R. Petersen, P. Frost, J.J. Gibbons, H. Wu and C.L. Sawyers, Enhanced sensitivity of PTEN-deficient tumors to inhibition of FRAP/mTOR, Proc. Natl. Acad. Sci. U. S. A. 98 (2001), pp. 10314–10319. Cerca con Google

82. Nho RS, Xia H, Diebold D, Kahm J, Kleidon J, White E, Henke CA. Cerca con Google

PTEN regulates fibroblast elimination during collagen matrix contraction. Cerca con Google

J Biol Chem. 2006 Nov 3;281(44):33291-301. Cerca con Google

83. Okamura H., K. Yoshida, H. Morimoto and T. Haneji, PTEN expression elicited by EGR-1 transcription factor in calyculin A-induced apoptotic cells, J. Cell. Biochem. 94 (1) (2005), pp. 117–125. Cerca con Google

84. Okubo T, Hogan BL. Hyperactive Wnt signaling changes the developmental potential of embryonic lung endoderm. J Biol 3: 11, 2004. Cerca con Google

85. Pap M. and G.M. Cooper, Role of glycogen synthase kinase-3 in the phosphatidylinositol 3-Kinase/Akt cell survival pathway, J. Biol. Chem. 273 (1998), pp. 19929–19932. Cerca con Google

86. Paramio JM, Navarro M, Segrelles C, Gomez-Casero E, Jorcano JL. PTEN tumour suppressor is linked to the cell cycle control through the retinoblastoma protein. Oncogene 1999;18:7462–7468. Cerca con Google

87. Pene F., Y.E. Claessens, O. Muller, F. Viguie, P. Mayeux, F. Dreyfus, C. Lacombe and D. Bouscary, Role of the phosphatidylinositol 3-kinase/Akt and mTOR/P70S6-kinase pathways in the proliferation and apoptosis in multiple myeloma, Oncogene 21 (2002), pp. 6587–6597. Cerca con Google

88. Pepicelli, C. V., Lewis, P. M. and McMahon, A. P. (1998). Sonic hedgehog regulates branching morphogenesis in the mammalian lung. Curr. Biol. 8,1083 -1086. Cerca con Google

89. Perl, A. K., Wert, S. E., Nagy, A., Lobe, C. G. and Whitsett, J. A. (2002). Early restriction of peripheral and proximal cell lineages during formation of the lung. Proc. Natl. Acad. Sci. USA 99,10482 -10487. Cerca con Google

90. Podsypanina K., L.H. Ellenson, A. Nemes, J. Gu, M. Tamura, K.M. Yamada, C. Cordon-Cardo, G. Catoretti, P.E. Fisher and R. Parsons, Mutation of Pten/Mmac1 in mice causes neoplasia in multiple organ systems, Proc. Natl. Acad. Sci. U. S. A. 96 (1999), pp. 1563–1568. Cerca con Google

91. Polakis P., Wnt signaling and cancer, Genes Dev. 14 (2000), pp. 1837–1851. Cerca con Google

92. Potten, C. S. (2004). Development of epithelial stem cell concepts. In Handbook of Stem Cells: Vol. 2 Adult and Fetal Stems Cell (ed. R. Lanza), pp.1 -12. New York: Academic Press. Cerca con Google

93. Raff, M. (2003). Adult stem cell plasticity: fact or artifact? Annu. Rev. Cell Dev. Biol. 19, 1-22. Cerca con Google

94. Rasheed et al., 1997 B.K. Rasheed, T.T. Stenzel, R.E. McLendon, R. Parsons, A.H. Friedman, H.S. Friedman, D.D. Bigner and S.H. Bigner, PTEN gene mutations are seen in high-grade but not in low-grade gliomas, Cancer Res. 57 (1997), pp. 4187–4190. Cerca con Google

95. Rawlins, E. L. and Hogan, B. L. (2005). Intercellular growth factor signaling and the development of mouse tracheal submucosal glands. Dev. Dyn. 233,1378 -1385. Cerca con Google

96. Rawlins EL, Hogan BL. Epithelial stem cells of the lung: privileged few or opportunities for many? Development. 2006 Jul;133(13):2455-65. Cerca con Google

97. T. Reya, S.J. Morrison, M.F. Clarke and I.L. Weissman, Stem cells, cancer, and cancer stem cells, Nature 414 (2001), pp. 105–111. Cerca con Google

98. Rossant, J., Zirngibl, R., Cado, D., Shago, M. and Giguere, V. (1991). Expression of a retinoic acid response element-hsplacZ transgene defines specific domains of transcriptional activity during mouse embryogenesis. Genes Dev. 5,1333 -1344. Cerca con Google

99. Schoch, K. G., Lori, A., Burns, K. A., Eldred, T., Olsen, J. C. and Randell, S. H. (2004). A subset of mouse tracheal epithelial basal cells generates large colonies in vitro. Am. J. Physiol. Lung Cell Mol. Physiol. 286,L631 -L642. Cerca con Google

100. Serini G, Bochaton-Piallat ML, Ropraz P, Geinoz A, Borsi L, Zardi L, Cerca con Google

Gabbiani G. The ?bronectin domain ED-A is crucial for myo?broblastic phenotype induction by transforming growth factor-beta1. J Cell Biol 1998;142:873–881. Cerca con Google

101. Serls, A. E., Doherty, S., Parvatiyar, P., Wells, J. M. and Deutsch, G. H. (2005). Different thresholds of fibroblast growth factors pattern the ventral foregut into liver and lung. Development 132,35 -47. Cerca con Google

102. Shu, W., Jiang, Y. Q., Lu, M. M. and Morrisey, E. E. (2002). Wnt7b regulates mesenchymal proliferation and vascular development in the lung. Development 129,4831 -4842. Cerca con Google

103. Shu W, Guttentag S, Wang Z, Andl T, Ballard P, Lu MM, Piccolo S, Birchmeier W, Whitsett JA, Millar SE, Morrisey EE. Wnt/beta-catenin signaling acts upstream of N-myc, BMP4, FGF signaling to regulate proximal-distal patterning in the lung. Dev Biol 283: 226–239, 2005. Cerca con Google

104. Song K., S.C. Cornelius, M. Reiss and D. Danielpour, Insulin-like growth factor-I inhibits transcriptional responses of transforming growth factor-beta by phosphatidylinositol 3-kinase/Akt-dependent suppression of the activation of Smad3 but not Smad2, J. Biol. Chem. 278 (2003), pp. 38342–38351. Cerca con Google

105. Stafford, D. and Prince, V. E. (2002). Retinoic acid signaling is required for a critical early step in zebrafish pancreatic development. Curr. Biol. 12,1215 -1220. Cerca con Google

106. Stambolic V, Suzuki A, de la Pompa JL, Brothers GM, Mirtsos C, Sasaki T, Ruland J, Penninger JM, Siderovski DP, Mak TW. Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell 1998;95:29–39. Cerca con Google

107. Stiles B., V. Gilman, N. Khanzenzon, R. Lesche, A. Li, R. Qiao, X. Liu and H. Wu, Essential role of AKT-1/protein kinase B alpha in PTEN-controlled tumorigenesis, Mol. Cell. Biol. 22 (2002), pp. 3842–3851. Cerca con Google

108. Stiles B., M. Groszer, S. Wang, J. Jiao and H. Wu, PTENless means more, Dev. Biol. 273 (2) (2004), pp. 175–184 15. Cerca con Google

109. Stillwell WG, Horning MG, Griffin GW, Tsang WS. Identification and synthesis of the isomeric tetrahydroxytetrahydronaphthalene metabolites excreted in rat urine. Drug Metab Dispos. 1982 Jan-Feb;10(1):11-4. Cerca con Google

110. Stokoe D., PTEN, Curr. Biol. 11 2001, p. R502 Cerca con Google

111. Stripp B, R, Maxson K, Mera R, Singh G.Plasticity of airway cell proliferation and gene expression after acute naphthalene injury. Am J Physiol. 1995 Dec;269(6 Pt 1):L791-9. Cerca con Google

112. Suganuma H, Sato A, Tamura R, Chida K. Enhanced migration of ?bro- Cerca con Google

blasts derived from lungs with ?brotic lesions. Thorax 1995;50:984–989. Cerca con Google

113. Sun H., R. Lesche, D.M. Li, J. Liliental, H. Zhang, J. Gao, N. Gavrilova, B. Mueller, X. Liu and H. Wu, PTEN modulates cell cycle progression and cell survival by regulating phosphatidylinositol 3,4,5,-trisphosphate and Akt/protein kinase B signaling pathway, Proc. Natl. Acad. Sci. U. S. A. 96 (1999), pp. 6199–6204. Cerca con Google

114. A. Suzuki, J.L. de la Pompa, V. Stambolic, A.J. Elia, T. Sasaki, I. del Barco Barrantes, A. Ho, A. Wakeham, A. Itie, W. Khoo, M. Fukumoto and T.W. Mak, High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice, Curr. Biol. 8 (1998), pp. 1169–1178. Cerca con Google

115. Suzuki A., S. Itami, M. Ohishi, K. Hamada, T. Inoue, N. Komazawa, H. Senoo, T. Sasaki, J. Takeda, M. Manabe, T.W. Mak and T. Nakano, Keratinocyte-specific Pten deficiency results in epidermal hyperplasia, accelerated hair follicle morphogenesis and tumor formation, Cancer Res. 63 (2003), pp. 674–681. Cerca con Google

116. Tebar M, Destree O, de Vree WJ, Ten Have-Opbroek AA. Expression of Tcf/Lef and sFrp and localization of beta-catenin in the developing mouse lung. Mech Dev 109: 437–440, 2001. Cerca con Google

117. Verdu J., M.A. Buratovich, E.L. Wilder and M.J. Birnbaum, Cell-autonomous regulation of cell and organ growth in Drosophila by Akt/PKB, Nat. Cell Biol. 1 (1999), pp. 500–506. Cerca con Google

118. Vivanco I. and C.L. Sawyers, The phosphatidylinositol 3-Kinase AKT pathway in human cancer, Nat. Rev., Cancer 2 (2002), pp. 489–501. Cerca con Google

119. Wan, H., Dingle, S., Xu, Y., Besnard, V., Kaestner, K. H., Ang, S. L., Wert, S., Stahlman, M. T. and Whitsett, J. A. (2005). Compensatory Roles of Foxa1 and Foxa2 during Lung Morphogenesis. J. Biol. Chem. 280,13809 -13816. Cerca con Google

120. Wan H, Xu Y, Ikegami M, Stahlman MT, Kaestner KH, Ang SL, Whitsett JA. Foxa2 is required for transition to air breathing at birth. Proc Natl Acad Sci USA 101: 14449–14454, 2004. Cerca con Google

121. Wang S., J. Gao, Q. Lei, N. Rozengurt, C. Pritchard, J. Jiao, G.V. Thomas, G. Li, P. Roy-Burman, P.S. Nelson, X. Liu and H. Wu, Prostate-specific deletion of the murine Pten tumor suppressor gene leads to metastatic prostate cancer, Cancer Cell 4 (2003), pp. 209–221. Cerca con Google

122. Warburton D, Perin L, Defilippo R, Bellusci S, Shi W, Driscoll B. Stem/progenitor cells in lung development, injury repair, and regeneration. Proc Am Thorac Soc. 2008 Aug 15;5(6):703-6. Cerca con Google

123. Wells, J. M. and Melton, D. A. (1999). Vertebrate endoderm development. Annu. Rev. Cell Dev. Biol. 15,393 -410. Cerca con Google

124. Weaver, M., Batts, L. and Hogan, B. L. (2003). Tissue interactions pattern the mesenchyme of the embryonic mouse lung. Dev. Biol. 258,169 -184. Cerca con Google

125. Wert, S. E., Glasser, S. W., Korfhagen, T. R. and Whitsett, J. A. (1993). Transcriptional elements from the human SP-C gene direct expression in the primordial respiratory epithelium of transgenic mice. Dev. Biol. 156,426 -44. Cerca con Google

126. Wert SE, Dey CR, Blair PA, Kimura S, Whitsett JA. Increased expression of thyroid transcription factor-1 (TTF-1) in respiratory epithelial cells inhibits alveolarization and causes pulmonary inflammation. Dev Biol 242: 75–87, 2002. Cerca con Google

127. White ES, Atrasz RG, Hu B, Phan SH, Stambolic V, Mak TW, Hogaboam CM, Flaherty KR, Martinez FJ, Kontos CD, Toews GB. Negative regulation of myofibroblast differentiation by PTEN (Phosphatase and Tensin Homolog Deleted on chromosome 10). Am J Respir Crit Care Med. 2006 Jan 1;173(1):112-21. Cerca con Google

128. White ES, Thannickal VJ, Carskadon SL, Dickie EG, Livant DL, Markwart S, Toews GB, Arenberg DA. Integrin {alpha}4{beta}1 regulates migration across basement membranes by lung fibroblasts: a role for phosphatase and tensin homologue deleted on chromosome 10. Am J Respir Crit Care Med 2003;168:436–442. Cerca con Google

129. Wolfrum C., D. Besser, E. Luca and M. Stoffel, Insulin regulates the activity of forkhead transcription factor Hnf-3beta/Foxa-2 by Akt-mediated phosphorylation and nuclear/cytosolic localization, Proc. Natl. Acad. Sci. U. S. A. 100 (2003), pp. 11624–11629. Cerca con Google

130. Wood, H. B., May, G., Healy, L., Enver, T. and Morriss-Kay, G. M. (1997). CD34 expression patterns during early mouse development are related to modes of blood vessel formation and reveal additional sites of hematopoiesis. Blood 90,2300 -2311. Cerca con Google

131. Wu X., K. Senechal, M.S. Neshat, Y.E. Whang and C.L. Sawyers, The PTEN/MMAC1 tumor suppressor phosphatase functions as a negative regulator of the phosphoinositide 3-kinase/Akt pathway, Proc. Natl. Acad. Sci. U. S. A. 95 (1998), pp. 15587–15591. Cerca con Google

132. Xing Y, Li C, Hu L, Tiozzo C, Li M, Chai Y, Bellusci S, Anderson S, Minoo P. Mechanisms of TGFbeta inhibition of LUNG endodermal morphogenesis: the role of TbetaRII, Smads, Nkx2.1 and Pten. Dev Biol. 2008 Aug 15;320(2):340-50. Cerca con Google

133. Xu Q, Tam M, Anderson SA. Fate mapping Nkx2.1-lineage cells in the mouse telencephalon. J Comp Neurol. 2008 Jan 1;506(1):16-29. Cerca con Google

134. Yanagi S, Kishimoto H, Kawahara K, Sasaki T, Sasaki M, Nishio M, Yajima N, Hamada K, Horie Y, Kubo H, Whitsett JA, Mak TW, Nakano T, Nakazato M, Suzuki A. Pten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice. J Clin Invest. 2007 Oct;117(10):2929-40. Cerca con Google

135. Yost C., M. Torres, J.R. Miller, E. Huang, D. Kimelman and R.T. Moon, The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3, Genes Dev. 10 (1996), pp. 1443–1454 Cerca con Google

136. Zhang J, Grindley JC, Yin T, Jayasinghe S, He XC, Ross JT, Haug JS, Rupp D, Porter-Westpfahl KS, Wiedemann LM, Wu H, Li L. PTEN maintains haematopoietic stem cells and acts in lineage choice and leukemia prevention. Nature. 2006 May 25;441(7092):518-22. Cerca con Google

137. Zhang S, Smartt H, Holgate ST, Roche WR. Growth factors secreted by bronchial epithelial cells control myofibroblast proliferation: an in vitro co-culture model of airway remodeling in asthma. Lab Invest 1999;79:395–405. Cerca con Google

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