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Simonato, Manuela (2009) Applicazioni biomediche di tecniche di spettrometria di massa. [Ph.D. thesis]

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Abstract (english)

The aim of this thesis concerning the setting of new methods to study methabolic pathaways in vivo using both stable isotope tracer (113C-leucine and D2O) both stable isotope at natural abundance.
First chapter reports a new method to measure absolute and percentage of AA and DHA synthesis. Preterm infants were fed with a formula enriched in AA and DHA with a higher content of 13C than did the endogeusly sinthesized long chain fatty acids (LCP).
The second chapter reports a new menthod to measure SP-B synthesis in vivo in humans by means of stable isotopes. We administered a 24 h infusion of 13C-Leucine as
metabolic precursor of SP-B and 2H2O as precursor of dipalmitoyl phosphatidylcholine (DSPC).

Abstract (italian)

Obiettivo di questa tesi è stato l’applicazione sia di isotopi stabili ad abbondanze naturali sia di traccianti marcati con isotopi stabili (113C-leucina e D2O) per lo studio di diverse vie metaboliche, quali la sintesi, di acidi grassi, nel bambino pretermine e la sintesi proteica e lipidica nell’adulto.
Il primo studio ha visto l’utilizzo di una dieta arricchita con due acidi grassi che presentavano un’abbondanza naturalmente diversa di 13C rispetto a quella degli altri acidi grassi per determinare, per un lungo periodo di tempo, la sintesi assoluta e in percentuale di questi due acidi grassi nei bambini pretermine.
Nel secondo studio abbiamo utilizzato leucina marcata con 13C e acqua marcata con deuterio per determinare il metabolismo della fosfatidilcolina disatura (DSPC) e della proteina specifica B del surfattante (SP-B).

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EPrint type:Ph.D. thesis
Tutor:Cogo, Paola
Ph.D. course:Ciclo 21 > Scuole per il 21simo ciclo > MEDICINA DELLO SVILUPPO E SCIENZE DELLA PROGRAMMAZIONE > MALATTIE RARE
Data di deposito della tesi:30 January 2009
Anno di Pubblicazione:30 January 2009
Key Words:Surfattante, isotopi stabili, PUFA, SP-B
Settori scientifico-disciplinari MIUR:Area 06 - Scienze mediche > MED/10 Malattie dell'apparato respiratorio
Struttura di riferimento:Dipartimenti > pre 2012 - Dipartimento di Pediatria
Codice ID:1798
Depositato il:30 Jan 2009
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