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Gringeri, Enrico (2008) Un nuovo sistema di perfusione parenchimale per la preservazione del graft epatico per trapianto: valutazione sperimentale sul piccolo e grande animale. [Tesi di dottorato]

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Abstract (inglese)


In liver transplantation one of the most important promblem which has not a solution yet is the large discrepancy between supply and demand for liver transplantation. To expand the pool of organs we should find the way to utilize marginal livers like the Non-heart-beating-donors ones and the fatty grafts. The use of these livers is associated with high incidence of post-operative primary dysfunction caused by the damage due to the Cold Storage preservation in the context of the process of ischaemia- reperfusion in extreme hypothermia (4° C). The utilization of new methods in the conservation of hepatic grafts like Machine Perfusion becomes necessary, especially for its ability to reduce the damage of ischaemia-reperfusion in hypothermia.
The great potentiality of this Machine in sane and marginal organ preservation and the number of works which took place about it, stimulated us to the deepen this argument.


We decided to create a experimental model of Machine Perfusion for pigs and to test it with different temperatures to obtain the best condition of perfusion and graft preservation. We based our study on the various works that can be find in literature and on the research developed by the Università di Pavia on rats.


We used in our experiments 15 Landrace pig of the weight of 22 kilos each. We divided them in three groups of study. Group C: after hepatectomy the pig liver was preserved in Cold Storage (4° C) for 8 hours. Group N: after hepatectomy the pig liver was perfused in Normothermic Machine Perfusion (37°C) for 8 hours. Group I: the pig liver was perfused in Moderate Hypothermic Machine (20°C). After the preservation, all the 15 livers were tested with a rewarming (37°C) of 2 hours. During the two hours of the experiment we performed samples of blood and biopsies and we also valuated the indocianine green clearance.


The results obtained by the histology, the functional test and biochemical levels of AST, ALT and LDH, in the moderate hypothermic perfusion prove the superiority of this machine .


It is concluded after these experiments the great ability of 20°C Machine Perfusion in pig's liver transplantation. We think that this method of preservation will be able to improve the pool of donors using NHBD livers and fatty livers. In future our research will deepen the problematic connected with the preservation of marginal organs, in particular of steatotic livers.

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Tipo di EPrint:Tesi di dottorato
Relatore:cillo, umberto
Data di deposito della tesi:Gennaio 2008
Anno di Pubblicazione:Gennaio 2008
Parole chiave (italiano / inglese):machine perfusion, normothermic, liver transplantation, ischemia-reperfusion injury
Settori scientifico-disciplinari MIUR:Area 06 - Scienze mediche > MED/18 Chirurgia generale
Struttura di riferimento:Dipartimenti > pre 2012 - Dipartimento di Scienze Chirurgiche Gastroenterologiche "Pier Giuseppe Cevese"
Codice ID:590
Depositato il:09 Ott 2008
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1.Kupiec-Weglinski JW, Busuttil RW. Ischemia and reperfusion injury in liver transplantation. Transplant Proc. 2005 May;37(4):1653-6. Cerca con Google

2. McKeown CM, Edwards V, Phillips MJ, Harvey PR, Petrunka CN, Strasberg SM. Sinusoidal lining cell damage: the critical injury in cold preservation of liver allografts in the rat. Transplantation. 1988 Aug;46(2):178-91. Cerca con Google

3. Chazouilleres O, Calmus Y, Vaubourdolle M, Ballet F. Preservation-induced liver injury. Clinical aspects, mechanisms and therapeutic approaches. J Hepatol. 1993 Apr;18(1):123-34. Review. Cerca con Google

4. Smedsrod B, De Bleser PJ, Braet F, Lovisetti P, Vanderkerken K, Wisse E, Geerts A. Cerca con Google

Cell biology of liver endothelial and Kupffer cells. Gut. 1994 Nov;35(11):1509-16. Review. Cerca con Google

5. Takei Y, Gao WS, Hijioka T, Savier E, Lindert KA, Lemasters JJ, Thurman RG. Increase in survival of liver grafts after rinsing with warm Ringer's solution due to improvement of hepatic microcirculation. Transplantation. 1991 Aug;52(2):225-30. Cerca con Google

6. Takei Y, Marzi I, Gao WS, Gores GJ, Lemasters JJ, Thurman RG. Leukocyte adhesion and cell death following orthotopic liver transplantation in the rat. Transplantation. 1991 May;51(5):959-65. Cerca con Google

7. Cywes R, Mullen JB, Stratis MA, Greig PD, Levy GA, Harvey PR, Strasberg SM. Prediction of the outcome of transplantation in man by platelet adherence in donor liver allografts. Evidence of the importance of prepreservation injury. Transplantation. 1993 Aug;56(2):316-23. Cerca con Google

8. Takei Y, Marzi I, Kauffman FC, Currin RT, Lemasters JJ, Thurman RG. Increase in survival time of liver transplants by protease inhibitors and a calcium channel blocker, nisoldipine. Transplantation. 1990 Jul;50(1):14-20. Cerca con Google

9.Liang J, Yamaguchi Y, Matsumura F, Goto M, Akizuki E, Matsuda T, Okabe K, Ohshiro H, Ishihara K, Yamada S, Mori K, Ogawa M. Calcium-channel blocker attenuates Kupffer cell production of cytokine-induced neutrophil chemoattractant following ischemia-reperfusion in rat liver. Dig Dis Sci. 2000 Jan;45(1):201-9. Cerca con Google

10. Hijioka T, Rosenberg RL, Lemasters JJ, Thurman RG. Kupffer cells contain voltage-dependent calcium channels. Mol Pharmacol. 1992 Mar;41(3):435-40. Cerca con Google

11. Colletti LM, Remick DG, Burtch GD, Kunkel SL, Strieter RM, Campbell DA Jr. Role of tumor necrosis factor-alpha in the pathophysiologic alterations after hepatic ischemia/reperfusion injury in the rat. J Clin Invest. 1990 Jun;85(6):1936-43. Cerca con Google

12. Goto M, Takei Y, Kawano S, Tsuji S, Fukui H, Fushimi H, Nishimura Y, Kashiwagi T, Fusamoto H, Kamada T. Tumor necrosis factor and endotoxin in the pathogenesis of liver and pulmonary injuries after orthotopic liver transplantation in the rat. Hepatology. 1992 Aug;16(2):487-93. Cerca con Google

13. Wanner GA, Muller PE, Ertel W, Bauer M, Menger MD, Messmer K. Differential effect of anti-TNF-alpha antibody on proinflammatory cytokine release by Kupffer cells following liver ischemia and reperfusion. Shock. 1999 Jun;11(6):391-5. Cerca con Google

14. Currin RT, Reinstein LJ, Lichtman SN, Thurman RG, Lemasters JJ. Inhibition of tumor necrosis factor release from cultured rat Kupffer cells by agents that reduce graft failure from storage injury. Transplant Proc. 1993 Feb;25(1 Pt 2):1631-2. Cerca con Google

15. Greig PD, Woolf GM, Sinclair SB, Abecassis M, Strasberg SM, Taylor BR, Blendis LM, Superina RA, Glynn MF, Langer B, et al. Treatment of primary liver graft nonfunction with prostaglandin E1. Transplantation. 1989 Sep;48(3):447-53. Cerca con Google

16. Henley KS, Lucey MR, Normolle DP, Merion RM, McLaren ID, Crider BA, Mackie DS, Shieck VL, Nostrant TT, Brown KA, et al. A double-blind, randomized, placebo-controlled trial of prostaglandin E1 in liver transplantation. Hepatology. 1995 Feb;21(2):366-72. Cerca con Google

17.Kornberg A, Schotte U, Kupper B, Hommann M, Scheele J. Impact of selective prostaglandin E1 treatment on graft perfusion and function after liver transplantation. Cerca con Google

Hepatogastroenterology. 2004 Mar-Apr;51(56):526-31. Cerca con Google

18. Jaeschke H, Bautista AP, Spolarics Z, Spitzer JJ. Superoxide generation by neutrophils and Kupffer cells during in vivo reperfusion after hepatic ischemia in rats. J Leukoc Biol. 1992 Oct;52(4):377-82. Cerca con Google

19. Rentsch M, Puellmann K, Sirek S, Iesalnieks I, Kienle K, Mueller T, Bolder U, Geissler E, Jauch KW, Beham A. Benefit of Kupffer cell modulation with glycine versus Kupffer cell depletion after liver transplantation in the rat: effects on postischemic reperfusion injury, apoptotic cell death graft regeneration and survival*. Transpl Int. 2005 Sep;18(9):1079-89. Cerca con Google

20. Roland CR, Naziruddin B, Mohanakumar T, Flye MW. Gadolinium blocks rat Kupffer cell calcium channels: relevance to calcium-dependent prostaglandin E2 synthesis and septic mortality. Hepatology. 1999 Mar;29(3):756-65. Cerca con Google

21. Fitzpatrick DB, Karmazyn M. Comparative effects of calcium channel blocking agents and varying extracellular calcium concentration on hypoxia/reoxygenation and ischemia/reperfusion-induced cardiac injury. J Pharmacol Exp Ther. 1984 Mar;228(3):761-8. Cerca con Google

22. Wilson DR, Arnold PE, Burke TJ, Schrier RW. Mitochondrial calcium accumulation and respiration in ischemic acute renal failure in the rat. Kidney Int. 1984 Mar;25(3):519-26. Cerca con Google

23. Grinyo JM. Reperfusion injury. Transplant Proc. 1997 Feb-Mar;29(1-2):59-62. Review. Cerca con Google

24. Lemasters JJ, DiGuiseppi J, Nieminen AL, Herman B. Blebbing, free Ca2+ and mitochondrial membrane potential preceding cell death in hepatocytes. Nature. 1987 Jan 1-7;325(6099):78-81. Cerca con Google

25. Lemasters JJ, Ji S, Thurman RG. Centrilobular injury following hypoxia in isolated, perfused rat liver. Science. 1981 Aug 7;213(4508):661-3. Cerca con Google

26. Lemasters JJ, Stemkowski CJ, Ji S, Thurman RG. Cell surface changes and enzyme release during hypoxia and reoxygenation in the isolated, perfused rat liver. J Cell Biol. 1983 Sep;97(3):778-86. Cerca con Google

27. Cywes R, Greig PD, Sanabria JR, Clavien PA, Levy GA, Harvey PR, Strasberg SM. Effect of intraportal glucose infusion on hepatic glycogen content and degradation, and outcome of liver transplantation. Ann Surg. 1992 Sep;216(3):235-46; discussion 246-7. Cerca con Google

28. Morgan GR, Sanabria JR, Clavien PA, Phillips MJ, Edwards C, Harvey PR, Strasberg SM. Correlation of donor nutritional status with sinusoidal lining cell viability and liver function in the rat. Transplantation. 1991 Jun;51(6):1176-83. Cerca con Google

29. Boudjema K, Lindell SL, Southard JH, Belzer FO. The effects of fasting on the quality of liver preservation by simple cold storage. Transplantation. 1990 Dec;50(6):943-8. Cerca con Google

30. Palombo JD, Hirschberg Y, Pomposelli JJ, Blackburn GL, Zeisel SH, Bistrian BR. Decreased loss of liver adenosine triphosphate during hypothermic preservation in rats pretreated with glucose: implications for organ donor management. Gastroenterology. 1988 Oct;95(4):1043-9. Cerca con Google

31. Jaeschke H. Preservation injury: mechanisms, prevention and consequences. J Hepatol. 1996 Nov;25(5):774-80. Review. Cerca con Google

32. Sumimoto R, Southard JH, Belzer FO. Livers from fasted rats acquire resistance to warm and cold ischemia injury. Transplantation. 1993 Apr;55(4):728-32. Cerca con Google

33. Sankary HN, Chong A, Foster P, Brown E, Shen J, Kimura R, Rayudu G, Williams J. Inactivation of Kupffer cells after prolonged donor fasting improves viability of transplanted hepatic allografts. Hepatology. 1995 Oct;22(4 Pt 1):1236-42. Cerca con Google

34. Zwacka RM, Zhang Y, Halldorson J, Schlossberg H, Dudus L, Engelhardt JF. CD4(+) T-lymphocytes mediate ischemia/reperfusion-induced inflammatory responses in mouse liver. Cerca con Google

J Clin Invest. 1997 Jul 15;100(2):279-89. Cerca con Google

35. Jaeschke H, Farhood A, Smith CW. Neutrophils contribute to ischemia/reperfusion injury in rat liver in vivo. FASEB J. 1990 Dec;4(15):3355-9. Cerca con Google

36. Essani NA, Fisher MA, Farhood A, Manning AM, Smith CW, Jaeschke H. Cytokine-induced upregulation of hepatic intercellular adhesion molecule-1 messenger RNA expression and its role in the pathophysiology of murine endotoxin shock and acute liver failure. Hepatology. 1995 Jun;21(6):1632-9. Cerca con Google

37. Jaeschke H, Farhood A, Bautista AP, Spolarics Z, Spitzer JJ, Smith CW. Functional inactivation of neutrophils with a Mac-1 (CD11b/CD18) monoclonal antibody protects against ischemia-reperfusion injury in rat liver. Hepatology. 1993 May;17(5):915-23. Cerca con Google

38. Farhood A, McGuire GM, Manning AM, Miyasaka M, Smith CW, Jaeschke H. Intercellular adhesion molecule 1 (ICAM-1) expression and its role in neutrophil-induced ischemia-reperfusion injury in rat liver. J Leukoc Biol. 1995 Mar;57(3):368-74. Cerca con Google

39.Jaeschke H, Smith CW. Mechanisms of neutrophil-induced parenchymal cell injury. J Leukoc Biol. 1997 Jun;61(6):647-53. Review. Cerca con Google

40. Mandel LJ, Takano T, Soltoff SP, Murdaugh S. Mechanisms whereby exogenous adenine nucleotides improve rabbit renal proximal function during and after anoxia. J Clin Invest. 1988 Apr;81(4):1255-64. Cerca con Google

41. Kloner RA, Przyklenk K, Whittaker P. Deleterious effects of oxygen radicals in ischemia/reperfusion. Resolved and unresolved issues. Circulation. 1989 Nov;80(5):1115-27. Review. Cerca con Google

42. Rhoden E, Pereira-Lima L, Lucas M, Mauri M, Rhoden C, Pereira-Lima JC, Zettler C, Petteffi L, Bello-Klein A. The effects of allopurinol in hepatic ischemia and reperfusion: experimental study in rats. Eur Surg Res. 2000;32(4):215-22. Cerca con Google

43. Rauen U, Viebahn R, Lauchart W, de Groot H. The potential role of reactive oxygen species in liver ischemia/reperfusion injury following liver surgery. Hepatogastroenterology. 1994 Aug;41(4):333-6. Review. Cerca con Google

44. Yuzawa H, Fujioka H, Mizoe A, Azuma T, Furui J, Nishikawa M, Hashida M, Kanematsu T. Inhibitory effects of safe and novel SOD derivatives, galactosylated-SOD, on hepatic warm ischemia/reperfusion injury in pigs. Hepatogastroenterology. 2005 May- Jun;52(63):839-43. Cerca con Google

45. Bauer M, Bauer I. Heme oxygenase-1: redox regulation and role in the hepatic response to oxidative stress. Antioxid Redox Signal. 2002 Oct;4(5):749-58. Review. Cerca con Google

46. Gores GJ, Nieminen AL, Fleishman KE, Dawson TL, Herman B, Lemasters JJ. Extracellular acidosis delays onset of cell death in ATP-depleted hepatocytes. Am J Physiol. 1988 Sep;255(3 Pt 1):C315-22. Cerca con Google

47. Caldwell-Kenkel JC, Currin RT, Coote A, Thurman RG, Lemasters JJ. Reperfusion injury to endothelial cells after cold storage of rat livers: protection by mildly acidic pH and lack of protection by antioxidants. Transpl Int. 1995;8(2):77-85. Cerca con Google

48. Gunter TE, Wingrove DE, Banerjee S, Gunter KK. Mechanisms of mitochondrial calcium transport. Adv Exp Med Biol. 1988;232:1-14. Cerca con Google

49. Vollmar B, Glasz J, Leiderer R, Post S, Menger MD. Hepatic microcirculatory perfusion failure is a determinant of liver dysfunction in warm ischemia-reperfusion. Am J Pathol. 1994 Dec;145(6):1421-31. Cerca con Google

50. Wang Y, Mathews WR, Guido DM, Farhood A, Jaeschke H. Inhibition of nitric oxide synthesis aggravates reperfusion injury after hepatic ischemia and endotoxemia. Shock. 1995 Oct;4(4):282-8. Cerca con Google

51. Hanazaki K, Kuroda T, Haba Y, Shiohara E, Kajikawa S, Iida F. Protective effect of the thromboxane A2 receptor antagonist ONO 3708 on ischemia-reperfusion injury in the dog liver. Surgery. 1994 Nov;116(5):896-903. Cerca con Google

52. Goto M, Takei Y, Kawano S, Nagano K, Tsuji S, Masuda E, Nishimura Y, Okumura S, Kashiwagi T, Fusamoto H, et al. Endothelin-1 is involved in the pathogenesis of ischemia/reperfusion liver injury by hepatic microcirculatory disturbances. Hepatology. 1994 Mar;19(3):675-81. Cerca con Google

53. Southard JH., Pienaar BH., McAnulty JF.: The UW solution for organ preservation Trasplant rev.1989; 3:103-130 Cerca con Google

54. Sumimoto R, Kamada N. Lactobionate as the most important component in UW solution for liver preservation. Transplant Proc. 1990 Oct;22(5):2198-9. Cerca con Google

55. Vregdenhil PK, Belzer FO, Southard JH: Adenie stimulation of adenosine triphosphate synthesis in cold-stored hepatocytes. Trasplantation 1991 51:909-911 Cerca con Google

56. Boudjema K, Van Gulik TM, Lindell SL, Vreugdenhil PS, Southard JH, Belzer FO. Effect of oxidized and reduced glutathione in liver preservation. Transplantation. 1990 Dec;50(6):948-51. Cerca con Google

57. Nardo B, Bellusci R, de Badiali GL, Pasquinelli G, Ferlaino F, de Raffele E, Recordare A, Vivarelli M, Mazziotti A, Cavallari A, et al. Ultrastructural aspects of the iliac arteries of multiorgan donor preserved in University of Wisconsin solution for 48 hours. Transplant Proc. 1992 Oct;24(5):2077-8. Cerca con Google

58. Cavallari A, Nardo B, Pasquinelli G, Badiali de Giorgi L, Bellusci R, Ferlaino F, Recordare A, De Raffele E, Vivarelli M, Mazziotti A, et al. Subcellular lesions of the biliary tract in human liver transplants incurred during preservation. Transplant Proc. 1992 Oct;24(5):1979-80. Cerca con Google

59. Southard JH, Belzer FO. Organ preservation. Annu Rev Med. 1995;46:235-47. Review. Cerca con Google

60. Cavallari A, Cillo U, Nardo B, Filipponi F, Gringeri E, Montalti R, Vistoli F, D'amico F, Faenza A, Mosca F, Vitale A, D'amico D. A multicenter pilot prospective study comparing Celsior and University of Wisconsin preserving solutions for use in liver transplantation. Liver Transpl. 2003 Aug;9(8):814-21. Cerca con Google

61. Menasche P, Termignon JL, Pradier F, Grousset C, Mouas C, Alberici G, Weiss M, Piwnica A, Bloch G. Experimental evaluation of Celsior, a new heart preservation solution. Eur J Cardiothorac Surg. 1994;8(4):207-13. Cerca con Google

62. Belzer FO, Ashby BS, Dunphy JE. 24-hour and 72-hour preservation of canine kidneys. Lancet. 1967 Sep 9;2(7515):536-8. Cerca con Google

63. Bessems M, Doorschodt BM, van Vliet AK, van Gulik TM. Improved rat liver preservation by hypothermic continuous machine perfusion using polysol, a new, enriched preservation solution. Liver Transpl. 2005 May;11(5):539-46. Cerca con Google

64. Schon MR, Hunt CJ, Pegg DE, Wight DG. The possibility of resuscitating livers after warm ischemic injury. Transplantation. 1993 Jul;56(1):24-31. Cerca con Google

65. Barber WH, Hudson SL, Deierhoi MH, Laskow DA, Diethelm AG. Pulsatile perfusion preservation: early posttransplant dialysis requirement predicts rapid graft loss. Transplant Proc. 1990 Apr;22(2):446-7. Cerca con Google

66. Henry ML, Sommer BG, Tesi RJ, Ferguson RM. Improved immediate renal allograft function after initial simple cold storage. Transplant Proc. 1990 Apr;22(2):388-9. Cerca con Google

67. Friend PJ, Imber C, St Peter S, Lopez I, Butler AJ, Rees MA. Normothermic perfusion of the isolated liver. Transplant Proc. 2001 Nov-Dec;33(7-8):3436-8. Cerca con Google

68. Lee CY, Jain S, Duncan HM, Zhang JX, Jones JW Jr, Southard JH, Clemens MG. Survival transplantation of preserved non-heart-beating donor rat livers: preservation by hypothermic machine perfusion. Transplantation. 2003 Nov 27;76(10):1432-6. Cerca con Google

69. Jain S, Lee CY, Baicu S, Duncan H, Xu H, Jones JW Jr, Clemens MG, Brassil J, Taylor MJ, Brockbank KG. Hepatic function in hypothermically stored porcine livers: comparison of hypothermic machine perfusion vs cold storage. Transplant Proc. 2005 Jan-Feb;37(1):340-1. Cerca con Google

70. Matsuno N, Uchiyama M, Iwamoto H, Hama K, Narumi Y, Kikuchi K, Degawa H, Kozaki K, Nagao T. Machine perfusion preservation for liver transplantation from non-heart-beating donors with agonal stage. Transplant Proc. 2002 Nov;34(7):2610-1. Cerca con Google

71. Compagnon P, Clement B, Campion JP, Boudjema K. Effects of hypothermic machine perfusion on rat liver function depending on the route of perfusion. Transplantation. 2001 Aug 27;72(4):606-14. Cerca con Google

72. Kim JS, Boudjema K, D'Alessandro A, Southard JH. Machine perfusion of the liver: maintenance of mitochondrial function after 48-hour preservation. Transplant Proc. 1997 Dec;29(8):3452-4. Cerca con Google

73. Pienaar BH, Lindell SL, Van Gulik T, Southard JH, Belzer FO. Seventy-two-hour preservation of the canine liver by machine perfusion. Transplantation. 1990 Feb;49(2):258-60. Cerca con Google

74. van der Plaats A, 't Hart NA, Verkerke GJ, Leuvenink HG, Ploeg RJ, Rakhorst G. Hypothermic machine preservation in liver transplantation revisited: concepts and criteria in the new millennium. Ann Biomed Eng. 2004 Apr;32(4):623-31. Review. Cerca con Google

75. Imber CJ, St Peter SD, Lopez de Cenarruzabeitia I, Pigott D, James T, Taylor R, McGuire J, Hughes D, Butler A, Rees M, Friend PJ. Advantages of normothermic perfusion over cold storage in liver preservation. Transplantation. 2002 Mar 15;73(5):701-9. Cerca con Google

76. Lee CY, Zhang JX, Jones JW Jr, Southard JH, Clemens MG. Functional recovery of preserved livers following warm ischemia: improvement by machine perfusion preservation. Cerca con Google

Transplantation. 2002 Oct 15;74(7):944-51 Cerca con Google

77. Jain S, Xu H, Duncan H, Jones JW Jr, Zhang JX, Clemens MG, Lee CY. Ex-vivo study of flow dynamics and endothelial cell structure during extended hypothermic machine perfusion preservation of livers. Cryobiology. 2004 Jun;48(3):322-32. Cerca con Google

78. 't Hart NA, van der Plaats A, Leuvenink HG, van Goor H, Wiersema- Buist J, Verkerke GJ, Rakhorst G, Ploeg RJ. Hypothermic machine perfusion of the liver and the critical balance between perfusion pressures and endothelial injury. Transplant Proc. 2005 Jan-Feb;37(1):332-4. Cerca con Google

79. Fujita S, Hamamoto I, Nakamura K, Tanaka K, Ozawa K. Evaluation of oxygen necessity during hypothermic liver perfusion. Nippon Geka Hokan. 1993 Sep 1;62(5):228-40. Cerca con Google

80. Fujita S, Hamamoto I, Nakamura K, Tanaka K, Ozawa K. Isolated perfusion of rat livers: effect of temperature on O2 consumption, enzyme release, energy store, and morphology. Nippon Geka Hokan. 1993 Mar 1;62(2):58-70. Cerca con Google

81. Stubenitsky BM, Ametani M, Danielewicz R, Southard JH, Belzer FO. Regeneration of ATP in kidney slices after warm ischemia and hypothermic preservation. Transpl Int. 1995;8(4):293-7. Cerca con Google

82. Lockett CJ, Fuller BJ, Busza AL, Proctor E. Hypothermic perfusion preservation of liver: the role of phosphate in stimulating ATP synthesis studied by 31P NMR. Transpl Int. 1995;8(6):440-5. Cerca con Google

83. Doorschodt BM, Bessems M, van Vliet AK, van Gulik TM. The first disposable perfusion preservation system for kidney and liver grafts. Ann Transplant. 2004;9(2):40-1. Cerca con Google

84. Starnes HF Jr, Tewari A, Flokas K, Kosek JC, Brown D, Van-Kessel AL, Mondon CE. Effectiveness of a purified human hemoglobin as a blood substitute in the perfused rat liver. Cerca con Google

Gastroenterology. 1991 Nov;101(5):1345-53. Cerca con Google

85. Felker TE, Gantz D, Tercyak AM, Oliva C, Clark SB, Small DM. A comparison of lipoprotein secretion, bile production and hepatic morphology in isolated rat livers perfused with a perfluorocarbon emulsion or rat erythrocytes. Hepatology. 1991 Aug;14(2):340-51. Cerca con Google

86. Motoyama S, Saito S, Inaba H, Kitamura M, Minamiya Y, Suzuki H, Saito R, Kamata S, Nakae H, Ogawa J. Red blood cells attenuate sinusoidal endothelial cell injury by scavenging xanthine oxidase-dependent hydrogen peroxide in hyperoxic perfused rat liver. Liver. 2000 Jun;20(3):200-8. Cerca con Google

87. Bowers BA, Branum GD, Rotolo FS, Watters CR, Meyers WC. Bile flow--an index of ischemic injury. J Surg Res. 1987 May;42(5):565-9. Cerca con Google

88. Butler AJ, Rees MA, Wight DG, Casey ND, Alexander G, White DJ, Friend PJ. Successful extracorporeal porcine liver perfusion for 72 hr. Transplantation. 2002 Apr 27;73(8):1212-8. Cerca con Google

89. Schon MR, Kollmar O, Wolf S, Schrem H, Matthes M, Akkoc N, Schnoy NC, Neuhaus P. Liver transplantation after organ preservation with normothermic extracorporeal perfusion. Cerca con Google

Ann Surg. 2001 Jan;233(1):114-23. Cerca con Google

90. St Peter SD, Imber CJ, Lopez I, Hughes D, Friend PJ. Extended preservation of non-heart-beating donor livers with normothermic machine perfusion. Br J Surg. 2002 May;89(5):609-16. Cerca con Google

91. Imber CJ, St Peter SD, de Cenarruzabeitia IL, Lemonde H, Rees M, Butler A, Clayton PT, Friend PJ . Optimisation of bile production during normothermic preservation of porcine Cerca con Google

livers. Am J Transplant. 2002 Aug;2(7):593-9. Cerca con Google

92. Monbaliu D, Crabbe T, Roskams T, Fevery J, Verwaest C, Pirenne J. Livers from non-heart-beating donors tolerate short periods of warm ischemia. Transplantation. 2005 May 15;79(9):1226-30. Cerca con Google

93. Ikeda T, Yanaga K, Kishikawa K, Kakizoe S, Shimada M, Sugimachi K. Ischemic injury in liver transplantation: difference in injury sites between warm and cold ischemia in rats. Hepatology. 1992 Aug;16(2):454-61. Cerca con Google

94. Van der Hoeven JA, Lindell S, van Schilfgaarde R, Molema G, Ter HorstGJ, Cerca con Google

Southard JH, Ploeg RJ.Donor brain death reduces survival after transplantation in rat livers Cerca con Google

preserved for 20 hr. Transplantation. 2001 Nov 27;72(10):1632-6. Cerca con Google

95. van Der Hoeven JA, Ter Horst GJ, Molema G, de Vos P, Girbes AR, Postema F, Freund RL, Wiersema J, van Schilfgaarde R, Ploeg RJ. Effects of brain death and hemodynamic status on function and immunologic activation of the potential donor liver in the rat. Ann Surg. 2000 Dec;232(6):804-13. Cerca con Google

96. Casavilla A, Ramirez C, Shapiro R, Nghiem D, Miracle K, Fung JJ, Starzl TE. Experience with liver and kidney allografts from non-heart-beating donors. Transplant Proc. 1995 Oct;27(5):2898 Cerca con Google

97. Reich DJ, Munoz SJ, Rothstein KD, Nathan HM, Edwards JM, Hasz RD, Manzarbeitia CY. Controlled non-heart-beating donor liver transplantation: a successful single center experience, with topic update. Transplantation. 2000 Oct 27;70(8):1159-66. Cerca con Google

98. Otero A, Gomez-Gutierrez M, Suarez F, Arnal F, Fernandez-Garcia A, Aguirrezabalaga J, Garcia-Buitron J, Alvarez J, Manez R. Liver transplantation from maastricht category 2 non-heart-beating donors: a source to increase the donor pool? Transplant Proc. 2004 Apr;36(3):747-50. Cerca con Google

99. ROBBINS;et al. Le basi patologiche delle malattie.6a ed. 2 v. Padova, Piccin, 2000. Cerca con Google

100. D'Alessandro AM, Kalayoglu M, Sollinger HW, Hoffmann RM, Reed A, Knechtle SJ, Pirsch JD, Hafez GR, Lorentzen D, Belzer FO. The predictive value of donor liver biopsies on the development of primary nonfunction after orthotopic liver transplantation. Transplant Proc. 1991 Feb;23(1 Pt 2):1536-7. Cerca con Google

101. Ploeg RJ, D'Alessandro AM, Knechtle SJ, Stegall MD, Pirsch JD, Hoffmann RM, Sasaki T, Sollinger HW, Belzer FO, Kalayoglu M. Risk factors for primary dysfunction after liver transplantation—a multivariate analysis. Transplantation. 1993 Apr;55(4):807-13. Cerca con Google

102. Fishbein TM, Fiel MI, Emre S, Cubukcu O, Guy SR, Schwartz ME, Miller CM, Sheiner PA. Use of livers with microvesicular fat safely expands the donor pool. Transplantation. 1997 Jul 27;64(2):248-51. Cerca con Google

103. Hayashi M, Fujii K, Kiuchi T, Uryuhara K, Kasahara M, Takatsuki M, Takeichi T, Kitade H, Sugimoto T, Uemoto S, Asonuma K, Egawa H, Fujita S, Inomata Y, Tanaka K. Cerca con Google

Effects of fatty infiltration of the graft on the outcome of living-related liver transplantation. Transplant Proc. 1999 Feb-Mar;31(1-2):403. Cerca con Google

104. Sun B, Jiang HC, Piao DX, Qiao HQ, Zhang L. Effects of cold preservation and warm reperfusion on rat fatty liver. World J Gastroenterol. 2000 Apr;6(2):271-274. Cerca con Google

105. Hui AM, Kawasaki S, Makuuchi M, Nakayama J, Ikegami T, Miyagawa S. Liver injury following normothermic ischemia in steatotic rat liver. Hepatology. 1994 Nov;20(5):1287-93. Cerca con Google

106. Takeda Y, Arii S, Kaido T, Niwano M, Moriga T, Mori A, Hanaki K, Gorrin-Rivas MJ, Ishii T, Sato M, Imamura M. Morphologic alteration of hepatocytes and sinusoidal endothelial cells in rat fatty liver during cold preservation and the protective effect of hepatocyte growth factor. Transplantation. 1999 Mar 27;67(6):820-8. Cerca con Google

107. Husberg BS, Genyk YS, Klintmalm GB. A new rat model for studies of the ischemic injury after transplantation of fatty livers: improvement after postoperative administration of prostaglandin. Transplantation. 1994 Feb;57(3):457-8. Cerca con Google

108. D’Amico D.F., Bassi N., Tedeschi U., Cillo U. Liver Trasplantation, procedures and management. Milano, Masson, 1994 Cerca con Google

109. Cillo U., Vitale A. et Al. Liver trasplantation for the treatement of moderately and well-differentiated hepato carcinoma. Ann Surg. 2004 Feb; 23 (2): 150-9. Cerca con Google

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