Go to the content. | Move to the navigation | Go to the site search | Go to the menu | Contacts | Accessibility

| Create Account

Matkovic, Urska (2008) Construction of Adenoviral vectors for cancer gene therapy and evaluation of toxic effects of adenoviral infection on Adrenocortical cells. [Ph.D. thesis]

Full text disponibile come:

Documento PDF

Abstract (english)

Aims of the work were construction and evaluation of recombinant adenoviral vectors for gene therapy of esophageal, hepatocellular and adrenocortical carcinomas in vitro and in vivo. Since adenoviruses have tropism for adrenal gland, human adrenocortical cells were used to assess the toxicity of replication competent adenovirus type 5 and replication incompetent adenoviral vectors. In this regard, the effects of adenoviral infection on adrenocortical gene expression, cell proliferation, cell cycle, cell death and steroidogenesis were investigated.

Statistiche Download - Aggiungi a RefWorks
EPrint type:Ph.D. thesis
Tutor:Palù, Giorgio
Supervisor:Barzon, Luisa
Ph.D. course:Ciclo 20 > Corsi per il 20simo ciclo > VIROLOGIA E BIOTECNOLOGIE MICROBICHE
Data di deposito della tesi:31 January 2008
Anno di Pubblicazione:31 January 2008
Key Words:adenoviral vectors, adenovirus, cancer gene therapy, adrenal gland, steroidogenesis
Settori scientifico-disciplinari MIUR:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Struttura di riferimento:Dipartimenti > pre 2012 Dipartimento di Istologia, Microbiologia e Biotecnologie Mediche
Codice ID:678
Depositato il:24 Sep 2008
Simple Metadata
Full Metadata
EndNote Format


I riferimenti della bibliografia possono essere cercati con Cerca la citazione di AIRE, copiando il titolo dell'articolo (o del libro) e la rivista (se presente) nei campi appositi di "Cerca la Citazione di AIRE".
Le url contenute in alcuni riferimenti sono raggiungibili cliccando sul link alla fine della citazione (Vai!) e tramite Google (Ricerca con Google). Il risultato dipende dalla formattazione della citazione.

1. Somia N, Verma IM. Gene therapy: trials and tribulations. Nature Review Genetics 2000; 1: 91-99. Cerca con Google

2. Friedmann T. Overcoming the obstacles to gene therapy. Scientific American 1997; 276, 6: 96-101. Cerca con Google

3. Blaese RM. Gene therapy for cancer. Scientific American 1997; 276, 6: 111-115. Cerca con Google

4. McCornick F. Cancer gene therapy: fringe or cutting edge? Nature Review Cancer 2001; 1: 130-141. Cerca con Google

5. Barzon L, Boscaro M, Palù G. Endocrine aspects of cancer gene therapy. Endocrine Reviews 2004; 25, 1: 1-44. Cerca con Google

6. Luo D, Saltzman WM. Synthetic DNA delivery systems. Nature biotechnology 2000; 18: 33-37. Cerca con Google

7. Kay MA, Glorioso JC, Naldini L. Viral vectors for gene therapy: the art of turning infectious agents into vehicles of therapeutics. Nature Medicine 2001; 7, 1: 33- 40. Cerca con Google

8. Berk AJ. Adenoviridae: The viruses and their replication. Wold WSM, Horwitz MS. Adenoviruses. Fields Virology, 5th edition (LWW), 2006, 2356-2382; 2395- 2417. Cerca con Google

9. Russell WC. Update on adenovirus and its vectors. Journal of General Virology 2000; 81: 2573-2604. Cerca con Google

10. Bird LE, Ren J, Wright A, Leslie KD, Degreve B, Balzarini J, Stammers DK. Crystal structure of varicella zoster virus thymidine kinase. The Journal of Biological Chemistry 2003; 278, 27:24680-24687. Cerca con Google

11. Lusky M. Good Manufacturing Practice Production of Adenoviral Vectors for Clinical Trials. Human Gene Therapy 2005; 16:281–291. Cerca con Google

12. Benihoud K, Yeh P, Perricaudet M. Adenovirus vectors for gene delivery. Current Opinion in Biotechnology 1999; 10: 440-447. Cerca con Google

13. AdEasyTM Vector System. Application manual Version 1.4. 2002. Carlsbad, CA, Qbiogene,Inc. http://www.qbiogene.com/literature/protocols/geneexpression/pdf/m-adeasy.pdf (20. jan. 2004): 61 pp Vai! Cerca con Google

14. Anderson RD, Haskell RE, Xia H, Roessler BJ, Davidson BL. A simple method for the rapid generation of recombinant adenovirus vectors. Gene Therapy 2000; 7: 1034-1038. Cerca con Google

15. Bruning A, Runnebaum IB. CAR is a cell-cell adhesion protein in human cancer cells and is expressionally modulated by dexamethasone, TNF-? and TGF?. 2003; Gene Therapy 10: 198-205. Cerca con Google

16. Okegawa T, Pong RC, Li Y, Bergelson JM, Sagalowsky AI, Hsieh JT. The mechanism of the growth-inhibitory effect of coxsackie and adenovirus receptor (CAR) on human bladder cancer: a functional analysis of car protein structure. Cancer Research 2001; 61: 6592–6600. Cerca con Google

17. Seidman MA, Hogan SM, Wendland RL, Worgall S, Crystal RG, Leopold PL. Variation in adenovirus receptor expression and adenovirus vector-mediated transgene expression at defined stages of the cell cycle. Molecular Therapy 2001; 4: 13–21. Cerca con Google

18. Hemminki A, Kanerva A, Liu B, Wang M, Alvarez RD, Siegal GP et al. Modulation of coxsackie-adenovirus receptor expression for increased adenoviral transgene expression. Cancer Research 2003; 63: 847–853. Cerca con Google

19. Fechner H, Haack A, Wang H, Wang X, Eizema K, Pauschinger M et al. Expression of coxsackie adenovirus receptor and alphav-integrin does not correlate with adenovector targeting in vivo indicating anatomical vector barriers. Gene Therapy 1999; 6: 1520–1535. Cerca con Google

20. Alemany R, Curiel DT. CAR-binding ablation does not change biodistribution and toxicity of adenoviral vectors. Gene Therapy 2001; 8: 1347–1353. Cerca con Google

21. Martin K, Brie A, Saulnier P, Perricaudet M, Yeh P, Vigne E. Simultaneous CARand alpha V integrin-binding ablation fails to reduce Ad5 liver tropism. Molecular Therapy 2003; 8: 485–494. Cerca con Google

22. Smith T, Idamakanti N, Kylefjord H, Rollence M, King L, Kaloss M et al. In vivo hepatic adenoviral gene delivery occurs independently of the coxsackievirusadenovirus receptor. Molecular Therapy 2002; 5: 770–779. Cerca con Google

23. Nicklin SA, Wu E, Nemerow GR, Baker AH. The influence of adenovirus fiber structure and function on vector development for gene therapy. Molecular Therapy 2005; 12: 384–393. Cerca con Google

24. Smith TA, Idamakanti N, Rollence ML, Marshall-Neff J, Kim J, Mulgrew K et al. denovirus serotype 5 fiber shaft influences in vivo gene transfer in mice. Human Gene Therapy 2003; 14: 777–787. Cerca con Google

25. Shayakhmetov DM, Li ZY, Ni S, Lieber A. Analysis of adenovirus sequestration in the liver, transduction of hepatic cells, and innate toxicity after injection of fibermodified vectors. Journal of Virology 2004; 78: 5368–5381. Cerca con Google

26. Glasgow JN, Everts M, Curiel DT. Transductional targeting of adenovirus vectors for gene therapy. Cancer Gene Therapy 2006; 1-15. Cerca con Google

27. Douglas JT, Rogers BE, Rosenfeld ME, Michael SI, Feng M, Curiel DT. Targeted gene delivery by tropism-modified adenoviral vectors. Nature Biotechnologies 1996; 14: 1574–1578. Cerca con Google

28. Goldman CK, Rogers BE, Douglas JT, Sosnowski BA, Ying W, Siegal GP et al. Targeted gene delivery to Kaposi’s sarcoma cells via the fibroblast growth factor receptor. Cancer Research 1997; 57: 1447–1451. Cerca con Google

29. Gu DL, Gonzalez AM, Printz MA, Doukas J, Ying W, D’Andrea M et al. Fibroblast growth factor 2 retargeted adenovirus has redirected cellular tropism: evidence for reduced toxicity and enhanced antitumor activity in mice. Cancer Research 1999; 59: 2608–2614. Cerca con Google

30. Bauerschmitz GJ, Barker SD, Hemminki A. Adenoviral gene therapy for cancer: from vectors to targeted and replication competent agents (review). International journal of oncology 2002; 21: 1161–1174. Cerca con Google

31. Belousova N, Korokhov N, Krendelshchikova V, Simonenko V, Mikheeva G, Triozzi PL et al. Genetically targeted adenovirus vector directed to CD40- expressing cells. Journal of Virology 2003; 77: 11367–11377. Cerca con Google

32. Meulenbroek RA, Sargent KL, Lunde J, Jasmin BJ, Parks RJ. Use of adenovirus protein IX (pIX) to display large polypeptides on the virion-generation of fluorescent virus through the incorporation of pIX-GFP. Molecular Therapy 2004; 9: 617–624. Cerca con Google

33. Le LP, Everts M, Dmitriev IP, Davydova JG, Yamamoto M, Curiel DT. Fluorescently labelled adenovirus with pIX-EGFP for vector detection. Molecular Imaging 2004; 3: 105–116. Cerca con Google

34. Magnusson MK, Hong SS, Henning P, Boulanger P, Lindholm L. Genetic retargeting of adenovirus vectors: functionality of targeting ligands and their influence on virus viability. Journal of Gene Medicine 2002; 4: 356–370. Cerca con Google

35. Gonin P, Gaillard C. Gene transfer vector biodistribution: Pivotal safety studies in clinical gene therapy development. Gene Therapy 2004; 11 (Suppl. 1), S98- S108. Cerca con Google

36. Johnson M, Huyn S, Burton J, Sato M, and Wu L. Differential Biodistribution of Adenoviral Vector In Vivo as Monitored by Bioluminescence Imaging and Quantitative Polymerase Chain Reaction. Human Gene Therapy 2006; 17: 1262- 1269. Cerca con Google

37. Margolis G, Kilham L, Hoenig EM. Experimental adenovirus infection of the mouse adrenal gland. I. Light microscopic observations. American Journal of Pathology 1974; 75:363–74. Cerca con Google

38. Chao J, Zhang JJ, Lin KF, Chao L. Human kallikrein gene delivery attenuates hypertension, cardiac hypertrophy, and renal injury in Dahl salt-sensitive rats. Human Gene Therapy 1998; 9:21–31. Cerca con Google

39. Senoo M, Matsubara Y, Fujii K, Nagasaki Y, Hiratsuka M, Kure S et al. Adenovirus-mediated in utero gene transfer in mice and guinea pigs: tissue distribution of recombinant adenovirus determined by quantitative TaqManpolymerase chain reaction assay. Molecular genetics and metabolism 2000; 69:269–76. Cerca con Google

40. Groot-Wassink T, Aboagye EO, Glaser M, Lemoine NR, Vassaux G. Adenovirus biodistribution and non-invasive imaging of gene expression in vivo by positron emission tomography using the human sodium/iodide symporter as a reporter gene. Human Gene Therapy 2002;13:1723–35. Cerca con Google

41. Wang Y, Groot-Wassink T, Lemoine NR, and Vassaux G. Cellular characterization of the tropism of recombinant adenovirus for the adrenal glands. European Journal of Clinical Investigation 2003; 33, 794–798. Cerca con Google

42. Stauber E, Card C. Experimental intraamnionic exposure of bovine fetuses with subgroup 2, type 7 Adenovirus. Canadian Journal Comparative Medicine 1978; 42: 466-472. Cerca con Google

43. Cutlip RC, McClurkin AW. Leisons and pathogenesis of disease in young calves experimentally induced by a bovine adenovirus type 5 isolated from a calf with weak calf syndrome. American journal of veterinary research 1975; 36: 1095- 1098. Cerca con Google

44. Medvedev N, Shastina GV. Morphology of the adrenal cortex in infants with generalized adenovirus infections. Arkhiv patologii 1978; 40: 32-36. Cerca con Google

45. Alesci S, Ramsey WJ, Bornstein SR, Chrousos GP, Hornsby PJ, Benvenga S, Trimarchi F, Ehrhart-Bornstein M. Adenoviral vectors can impair adrenocortical steroidogenesis: clinical implications for natural infections and gene therapy. Proc Natl Acad Sci U S A. 2002;99:7484-9. Cerca con Google

46. Tajima T, Okada T, Ma XM, Ramsey W, Bornstein S, Aguilera G. Restoration of adrenal steroidogenesis by adenovirus-mediated transfer of human cytochrome P450 21-hydroxylase into the adrenal gland of 21-hydroxylase-deficient mice. Gene Therapy 1999; 6: 1898-1903. Cerca con Google

47. Ma L, Bluyssen HAR, Raeymaeker M, Laurysens V, Beek N, Pavliska H, Zonneveld AJ, Tomme P. Rapid determination of adenoviral vector titers by quantitative real-time PCR. Journal of Virological Methods 2001; 93: 181-188. Cerca con Google

48. Stilwell JL, Samulski JS. Role of viral vectors and virion shells in cellular gene expression. Molecular Therapy 2003; 9, 3. Cerca con Google

49. Wersto RP, Rosenthal ER, Seth PK, Eissa NT, Donahue RE. Recombinant, replication-defective adenovirus gene transfer vectors induce cell cycle dysregulation and inappropriate expression of cyclin proteins. Journal of Virology 1998; 9491-9502. Cerca con Google

50. Greco O, Dachs GU. Gene directed enzyme/prodrug therapy of cancer: historical appraisal and future prospectives. Journal of Cellular Physiology 2001; 187: 22- 36. Cerca con Google

51. Barzon L, Pacenti M, Boscaro M, Palù G. Gene therapy for thyroid cancer. Expert opinion on biological therapy 2004; 4:1225-1239. Cerca con Google

52. Brand K, Klocke R, Possling A, Paul D, Strauss M. Induction of apoptosis and G2/M arrest by infection with replication-deficient adenovirus at high multiplicity of infection. Gene Therapy 1999; 6:1054-63. Cerca con Google

53. Scibetta AG, Copier J, Barrett A, Chaplin T, Taylor-Papadimitriou J. Gene expression changes induced by a recombinant E1-/E3- adenovirus type 5 vector in human mammary epithelial cells. Intervirology 2005; 48: 350-61. Cerca con Google

54. Ramalingam R, Rafii S, Worgall S, Hackett NR, Crystal RG. Induction of endogenous genes following infection of human endothelial cells with an E1(-) E4(+) adenovirus gene transfer vector. Journal of Virology 1999; 73:10183-90. Cerca con Google

55. Clesham GJ, Adam PJ, Proudfoot D, Flynn PD, Efstathiou S, Weissberg PL: High adenoviral loads stimulate NF ?B-dependent gene expression in human vascular smooth muscle cells. Gene Therapy 1998; 5: 174–180. Cerca con Google

56. Lieber A, He CY, Meuse L, Schowalter D, Kirillova I, Winther B, Kay MA: The role of Kupffer cell activation and viral gene expression in early liver toxicity after infusion of recombinant adenovirus vectors. Journal of Virology 1997; 71: 8798– 8807. Cerca con Google

57. McElvaney NG, Crystal RG: IL-6 release and airway administration of human CFR cDNA adenovirus vector. Nature Medicine 1995; 1: 182–184. Cerca con Google

58. Zhao H, Granberg F, Elfi neh L, Pettersson U, Svensson C: Strategic attack on host cell gene expression during adenovirus infection. Journal of Virology 2003; 77: 11006–11015. Cerca con Google

59. Schnell MA, Zhang Y, Tazelaar J, Gao GP, Yu QC, Qian R, Chen SJ, Varnavski AN, LeClair C, Raper SE, Wilson JM. Activation of innate immunity in nonhuman primates following intraportal administration of adenoviral vectors. Molecular Therapy 2001; 3, 708-22. Cerca con Google

60. Zhang Y, Chirmule N, Gao GP, Qian R, Croyle M, Joshi B, Tazelaar J, Wilson JM. Acute cytokine response to systemic adenoviral vectors in mice is mediated by dendritic cells and macrophages. Molecular Therapy 2001; 3, 697-707. Cerca con Google

61. Driesse MJ, Esandi MC, Kros JM, Avezaat CJ, Vecht C, Zurcher C, van der Velde I, Valerio D, Bout A, Sillevis Smitt PA. Intra-CSF administered recombinant adenovirus causes an immune response-mediated toxicity. Gene Therapy 2000; 7, 1401-9. Cerca con Google

62. Liu Q, Zaiss AK, Colarusso P, Patel K, Haljan G, Wickham TJ, Muruve DA. The role of capsid-endothelial interactions in the innate immune response to adenovirus vectors. Human Gene Therapy 2003; 14:627-43. Cerca con Google

63. Zaiss AK, Liu Q, Bowen GP, Wong NC, Bartlett JS, Muruve DA. Differential activation of innate immune responses by adenovirus and adeno-associated virus vectors. Journal of Virology 2002; 76: 4580-90. Cerca con Google

64. Sargent CA, Dunham I, Trowsdale J, Campbell RD. Human major histocompatibility complex contains genes for the major heat shock protein HSP70. Proc Natl Acad Sci USA 1989; 86: 1968-1972. Cerca con Google

Download statistics

Solo per lo Staff dell Archivio: Modifica questo record