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Gallina, Guglielmo (2008) Marker urinari di trattamenti illeciti nel vitello: il residuo, i suoi metaboliti e nuovi parametri fisiologici. [Tesi di dottorato]

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Abstract (inglese)

In the EU the use of hormonal substances is prohibited in cattle fattening to avoid consumer exposure to unforeseeable risks from the intake of hormone residues and their metabolites. National Surveillance Plans for steroid abuse adopted the detection of the parent compound in urine and other sample matrices but, in spite of the progress in analytical techniques which allows to obtain very low detection limits for individual residues, this strategy may be unsuccessful; the use of natural hormones or of hormone precursors, the use of a combination of different active principles either at very low dosages or of unknown chemical structure are a few examples of procedures to escape the analytical controls. So effective surveillance and monitoring of the ban requires not only the availability of validated analysis methods for screening and confirmation of hormone residues in urine and other sample matrices but also knowledge about the absorption, biotransformation and excretion kinetics of illegally administered hormones, as well as endogenous levels of hormones in these sample matrices and also to develop complementary screening tests based on the biological action(s) of the substance(s) under investigation.
Aim of the thesis was to study the application of traditional analytical methods like LC/MS-MS , RIA and ELISA to detect to study the pattern of excretion of some urinary steroids in calves as marker of illicit treatments.
In the first part of this thesis is presented a preliminary assessment of physiological concentrations of testosterone (T) and epitestosterone (epiT) in urine of veal calves. Current methods to detect the misuse of natural androgens in cattle do not involve evaluation of urinary content of testosterone (T) because no validated physiological levels are set and threshold values cannot be fixed. In man urinary excretion of epiT occurs at similar or slightly lower concentration than that of T. Because of after exogenous T administration urinary epiT remains rather constant or decreases, while T increases, a threshold value of the ratio T/epiT = 6, in urine, was established as a limit for doping control in athletes. To evaluate whether urinary ratios could be useful for surveillance of exogenous hormone treatments also in calves urine samples were collected from control calves and from calves treated with oestradiol plus testosterone or oestradiol plus boldenone and urinary concentration of T and epiT was assessed by LC-MS/MS analysis. In all urine samples collected from control and treated animals the epiT concentrations were by far greater than T. EpiT may be considered one of the major urinary metabolites of T. Thus when the T/epiT ratio was calculated using the same approach adopted for doping control in man, no significant differences could be observed between treated and control calves. It is likely that the paramount difference between E and T concentrations masked the effect of treatment. Recent data, suggested that in calves repeated treatments with natural hormones could cause a time-course reduction of urinary E . Adopting the E/T ratio, the reduction of E urinary excretion can be confirmed in Testosterone treated calves (A) but still no evident in Boldenone treated ones.
The second part of this thesis deals with the identification of administation of a glucocorticoids like dexamethasone, (DXM) which is often illegally used as a growth promoters. To identify indirect biomarkers of illicit treatments, the urinary ratio between 6beta-hydroxycortisol (6beta-OHF) and cortisol (F), was measured in urines obtained from bulls experimentally treated per os and intramuscuarly (im) with different DXM dosages. Dexamethasone, given per os at low doses elicited an early and lasting significant reduction of 6beta-OHF/F. No significant variations were seen in urines from bulls given DXM intramuscularly. These results suggest 6beta-OHF/F as a rapid, non invasive, screening test for oral, low-dose, long-term corticosteroid treatment in cattle.
The last part regards the results of a study aiming to clarify the natural occurrence and the metabolism of boldenone in cattle.
The residue profiles of 17?-/17?-boldenone conjugated (17?/?-Bol) and ADD were investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in urine of male veal calves fed two commercial milk replacers, with different content of phytosterols and administered with 17?-Bol and ADD per os. The presence of conjugate 17?-Bol, ubiquitous and at higher concentrations in urine from control calves receiving the milk replacer with the greater amount of plant sterols support the theory that 17?-Bol is diet-related. In urine from treated animals the much higher17?-Bol levels were not modified by the production from diet precursors. The results confirmed the urinary excretion of 17?-Bol is due to exogenous administration of 17?-Bol. The discontinuous rate of elimination of both 17?- and 17?-Bol, despite the daily administration of 17?-Bol plus ADD, indicates the necessity for further research to detect other urinary boldenone metabolites to strength surveillance strategy.
To identify metabolites as in vivo markers to support the thesis of exogenous administration, a further approach to the in vitro biotransformation of 17?-Bol and ADD was performed using different subcellular fractions obtained from both liver and kidney of untreated cattle. Metabolites obtained from incubated parent compounds were separated by liquid chromatography (HPLC) elution and successively identified by mass spectrometry (LC-MS/MS) detection. The bovine liver was the target tissue of the main metabolic reaction transforming 17?-Bol to ADD and viceversa. The analysis of the incubation extracts detected thepresence of polar hydroxylated (m/z 303) derivates of 17?-Bol, 17?-Bol and ADD. The results indicated that the urinary excretion profile in vivo of 6?-hydroxy-17?-Bol, 16?-hydroxy-17?-Bol and 6?-hydroxy-17?-Bol could be studied together with 17?- and 17?-BOLD as putative markers of 17?-Bol treatment in cattle.
Preliminary results of investigation regarding the presence of the hydroxylated metabolites in urine of calves treated with boldenone shown, even though additional studies are in due course, candidate 6?-hydroxy-17?-Bol as new potential marker of anabolic abuse of boldenone.


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Tipo di EPrint:Tesi di dottorato
Relatore:Montesissa, Clara
Dottorato (corsi e scuole):Ciclo 20 > Scuole per il 20simo ciclo > SCIENZE VETERINARIE > SANITA' PUBBLICA VETERINARIA E PATOLOGIA COMPARATA
Data di deposito della tesi:30 Gennaio 2008
Anno di Pubblicazione:30 Gennaio 2008
Parole chiave (italiano / inglese):bovino, trattamenti illeciti
Settori scientifico-disciplinari MIUR:Area 07 - Scienze agrarie e veterinarie > VET/07 Farmacologia e tossicologia veterinaria
Struttura di riferimento:Dipartimenti > Dipartimento di Sanità pubblica, Patologia comparata ed Igiene veterinaria
Codice ID:778
Depositato il:11 Nov 2008
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1. Abraham G., Gottschalk J., Ungemach F.R. (2004) “Possible role of dexamethasone in sensitizing the beta-2-adrenergic receptor system in vivo in calves during concomitant treatment with clenbuterol” Pharmacology, 72, 196-204 Cerca con Google

2. Aguilera R., Hatton C.K., Catlin D.H. (2002) “Detection of epitestosterone doping by isotope ratio mass spectrometry” Clin. Chem., 48, 629-636 Cerca con Google

3. Angeletti R, Contiero L, Gallina G, Montesissa C. “The urinary ratio of testosterone to epitetosterone: a good marker of illegal treatment also in cattle?”. Veterinary Research Communications 2006; 30 Suppl 1:127-31 Cerca con Google

4. Arts C.J.M., Van Baak M.J., Den Hartog J.M.P. (1991) “Control system of the illegal use of naturally occuring steroids in calves” Journal of Chromatography, 546, 429-444 Cerca con Google

5. Arts C.J.M., Schilt R., Van Ginkel L.A. (1996) “Boldenone is a naturally occurring anabolic steroid in cattle” In Proceedings of the EuroResidue III, Haagsma, N., Ruiter, A.,Eds., Veldhoven, The Netherlands, 212-216 Cerca con Google

6. Baisch H., Otto U., Fack H. “Growth of human prostate carcinomas with and without hormone alpha- dehydrotestosterone in nude mice” Eur. Urol., 34, 505-512 Cerca con Google

7. Barthakur S., Roy M.K., Bera S.K., Ghosh A.C. (1996) “Steroid transformation by mutants of Mycobacterium sp. With altered response to antibiotics” J. Basic Microbiology, 36, 383-387 Cerca con Google

8. Batjoens P., De Brabander H.F., Smets F., Pottie G. (1994) Analyst, 119, 2607 Cerca con Google

9. Blokland M.H.; Van Rossum H.J.; Sterk S.S.; Van Ginkel L.A.; Stephany R.W. (2007) Development of a method which discriminates between endogenous and exogenous ?-boldenone Anal. Chim. Acta, 586, 147-153 Cerca con Google

10. Botsoglou A.N., Fletouris D.J. (2001) “Drug residues in foods: pharmacology, food safety and analysis” Ed. Marcel Dekker Inc. Cerca con Google

11. Cantiello M, Nachtmann C., Mastrone A., Carletti M., Virkel G., Nebbia C. (2006) “Biotransformation pattern of 17?-boldenone and of its precursor/metabolite androsta-1,4-diene-3,17-dione in bovine hepatic subfractions” J. vet. Pharmacol. Therap., 29 (suppl. 1), 168 Cerca con Google

12. Calvarese S., Rubini P., Urbani G., Ferri N. (1994) “Experimental administration of 19-nortestosterone and dexamethasone in cattle:elimination of the two drugs in different biological matrices.” Analyst, 119, 2611-26l5. Cerca con Google

13. Catlin D.H., Hatton C.K., Starcevic S.H. (1997). “Issue in detection abuse of xenobiotic anabolic steroids and testosterone by analysis of athletes’ urine”. Clin. Chem., 43, 1280-1288 Cerca con Google

14. Clouet A.S., Le Bizec B., Boerlen F., Monteau F., André F. (1998) “Calf primary hepatocyte culture as a tool for anabolic steroid metabolism studies” Analyst, 123, 2489-2492 Cerca con Google

15. Coldham N.G., Biancotto G., Montesissa C., Howells L.C., Sauer M.J. “Utility of isolated hepatocytes and radio-HPLC-MSn for the analysis of the metabolic fate of 19-nortestosterone laurate in cattle” Analyst, 123, 2589-2594 Cerca con Google

16. Conneely G., Aherne M., Lu H. e Guilbault G.G. (2007). “Development of an immunosensor for the detection of Testosterone in bovine urine” Anal. Chim. Acta, 583, 153-160 Cerca con Google

17. Contiero, B. Neri, L. Giannetti, R. Bagnati, G. Pozza, R. Angeletti, Proceedings of the Fifth International Symposium On Hormones and Veterinary Drug Residue Analysis, Antwerp, Belgium, May 16-19 2006, 71.. Cerca con Google

18. Courtheyn D., Le Bizec B., Brambilla G., De Brabander H.F., Cobbaert E., Van de Wiele M., Vercammen J., De Wasch K. (2002) “Recent devolpments in the use and abuse of growth promoters” Anal. Chim. Acta, 473, 71-82. Cerca con Google

19. Danhaive P.A., Rousseau G.G. (1986) “Binding of glucocorticoid antagonists to androgen and glucocorticoid hormone receptors in rat skeletal muscle” Journal of steroid biochemistry, 24, 482-487. Cerca con Google

20. De Brabander H.F., van Hande J. (1994) “Endogenic nortestosterone in cattle?” Analyst, 119, 2581-2582. Cerca con Google

21. De Brabander H.F., Poelmans S., Schilt R., Stephany R.W., Le Bizec B., Draisci R., Sterk S.S., Van Ginkel L.A., Courthney D., Van Hoof N., Macri A., De Wasch K. (2004) “Presence and metabolism of the anabolic steroid boldenone in various animal species: a review” Food Addit Contam, 21, 515-525. Cerca con Google

22. Daeseleire, E., De Guesquiere, A., Van Peteghem, C. (1993) Metabolism of 17?,19-nortestosterone in urine of calves after oral intake and intramuscular administration. Anal. Chim. Acta 275, 95-103. Cerca con Google

23. Dehenin L., Matsumoto A.M. (1993) “Long-term administration of testosterone enanthate to normal men: alternation of the urinary profile of androgen metabolites potentially useful for detection of testosterone misuse in sports” Journal of Steroid biochemistry and molecular biology, 44, 179-189. Cerca con Google

24. Donike M., Barwald K.R., Klostermann K., Schanzer W., Zimmermann J. (1983). “Detencion of exogenous testosterone” In: Heck H, Hollmann W, Liesen H, et al., eds. Sport: Leistung und Gesundheit, Kongressbd. Dtsch Sportarzte-kongress. Koln: Deutscher Arzte-Verlag, 293-8. Cerca con Google

25. Draisci R., Palleschi L., Ferretti E., Lucentini L., Cammarata P. (2000) “Quantitation of anabolic Hormones and their metabolites in bovine serum and urine by liquid chromatography-tandem mass spectrometry” Journal of chromatography A, 870, 511-522. Cerca con Google

26. Draisci R., Palleschi L., Ferretti E., Lucentini L., delli Quadri F. (2003) “Confirmatory analysis of 17?-boldenone, 17?-boldenone and androsta-1,4-diene-3,17-dione in bovine urine by liquid chromatography-tandem mass spectrometry” Journal of chromatography B, 789, 219-226. Cerca con Google

27. Draisci, R.; Merlanti, R.; Ferretti, G.; Fantozzi, L.; Ferranti, C.; Capolongo, F.; Segato, S.; Montesissa, C. (2007) “Excretion profile of boldenone in urine of veal calves fed two different milk replacers” Anal. Chim. Acta , 586, 171-176. Cerca con Google

28. El-Bahr S.M., Mostl E. And Palme R. (2003). “Glucocorticoid metabolites inhibit the metabolism of androstenedione in red blood cells of ruminants”. J. Vet. Med. A, 50, 98-102. Cerca con Google

29. El Desoky E.S., H.O. Mohamed, W.M.A. Farghaly, S.A. Hamed, M.A. Hedaya, J.P. Siest (2005) Pharmacol. Res. 51 575 Cerca con Google

30. Ferchaud V., Le Bizec B., Monteau F., André F. (1998) “Determination of the exogenous character of testosterone in bovine urine by gas chromatography-combustion-isotope ratio mass spectrometry” Analyst, 123, 2617-2620 Cerca con Google

31. Ferchaud V., Le Bizec B., Monteau F., Andre F. (2000) “Characterization of exogenous testosterone in livestock by gas chromatography/combustion/isotope ratio mass spectrometry: influence of feeding and age” Rapid. Commun. Mass Spectrom., 14, 652-656 Cerca con Google

32. Gaignage P., Lognay G., Bosson D., Vertongen D., Dreze P., Marlier M., Severin M. (1991) “Dexamethasone bovine pharmacokinetics” Eur. J. Drug Metab. Pharmacokinet., 16, 219-221 Cerca con Google

33. Galteau M.M., Shamsa F. (2003) “Urinary 6beta-hydroxycortisol: a validated test for evaluating drug induction or drug inhibition mediated through CYP3A in humans and in animals” Eur J Clin Pharmacol., 59, 713-733 Cerca con Google

34. Ged C., Rouillon J.M., Pichard L., Combalbert J., Bressot N., Bories P., Michel H., Beaune P., Maurel P. (1989) “The increase in urinary excretion of 6?-hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction” Br. J. Clin. Pharmacol., 28, 373 Cerca con Google

35. Groot M.J., Schilt R., Ossenkoppelf J.S., Berende P.L.M., Haasnoot W. (1998). “Combinations of growth promoters in veal calves: consequences for screening and confirmation methods” Journal of Veterinary Medicine 45, 425-440. Cerca con Google

36. Hagedorn H.W., Zankl H., Grund C., Schulz R. (1997) “Excretion of the anabolic steroid boldenone by racing pigeons” Am. J. Vet. Res., 58, 224-227. Cerca con Google

37. Hebestreit M., Flenker U., Buisson C., Andre F., Le Bizec B., Fry H., Lang M., Weigert A.P., Heinrich K., Hird S., Schanzer W. (2006). “Application of stable carbon isotope analysis to the detection of testosterone administration to cattle”. J Agric Food Chem., 54,2850-2858. Cerca con Google

38. Henricks D.M., Brandt R.T., Titgemeyer E.C. and Milton C.T. (1997) “Serum concentration of trenbolone-17? and estradiol-17? and performance of heifers treated with trenbolone acetate, melengestrol acetate, or estradiol-17?” J. Anim. Sci., 75, 2627-2633. Cerca con Google

39. Ho E.N,, Kwok W.H., Leung D.K., Wan T.S., Wong A.S. (2007) “Metabolic studies of turinabol in horses” Anal. Chim. Acta, 586, 208-216 Cerca con Google

40. Hooijerink D., Schilt R., Hoogenboom R., Huveneers-Oorsprong M. (1998) “Identification of metabolites of the anabolic steroid methandienone formed by bovine hepatocytes in vitro” Analyst; 123, 2637-2641 Cerca con Google

41. Illera J.C., Peña L., Martínez-Mateos M.M., Camacho L., Blass A., Garcia-Partida P.,Illera M.J., Silván G. (2007) “The effect of long-term exposure to combinations of growth promoters in Long Evans rats: part 2. Adrenal morphology (histopathology and immunochemical studies)” Anal. Chim. Acta, 586, 252-258 Cerca con Google

42. Impens S., Van Loco J., Degroodt J.M., De Brabander H. (2007) “A downscaled multi-residue strategy for detection of anabolic steroids in bovine urine using gas chromatography tandem mass spectrometry (GC-MS3)” Anal Chim Acta, 586, 43-48 Cerca con Google

43. Johnson B.J., Chung K.Y.(2007) “Alterations in the physiology of growth of cattle with growth-enhancing compounds” Vet Clin North Am Food Anim Pract., 23, 321-332 Cerca con Google

44. König T., Schuberth H.J., Leibold W., Zerbe H. (2006) “Dexamethasone depresses the expression of l-selectin but not the in vivo migration of bovine neutrophils into the uterus” Theriogenology, 65, 1227-1241 Cerca con Google

45. Labrie F., Luu-The V., Lin S.X., Labrie C., Simard J., Breton R., Bélanger A. (1997) “The key role of 17 beta-hydroxysteroid dehydrogenases in sex steroid biology” Steroids, 62, 148-58 Cerca con Google

46. Lake B.G. (1987) “Preparation and characterisation of microsomal fractions for studies on xenobiotic metabolism” Biochemicai Toxicology: A praticai approach, Sneil K. & MullocK B. IRL, Oxford University Press, Oxford, England. Cerca con Google

47. Le Bizec B., Montrade M.P., Monteau F., Andre F. (1993) “Detection and identification of anabolic steroids in bovine urine by gas chromatography-mass spectrometry” Analitica Chimica Acta, 275, 123-133. Cerca con Google

48. Le Bizec, B.; Courant, F.; Gaudin, I.; Bichon, E.; Destrez, B.; Schilt, R.; Draisci, R.; Monteau, F.; Andre, F. “Criteria to distinguish between natural situations and illegal use of boldenone, boldenone esters and boldione in cattle 1. Metabolite profiles of boldenone, boldenone esters and boldione in cattle urine” Steroids, 71, 1078-1087 Cerca con Google

49. Lowry O.H., Rosebrough N.Y., Farr A.L., Randali R.Y. (1951) “Protein measurament with the Folin phenol reagent” Journal of Biological Chemestry, 193, 265-275. Cerca con Google

50. Lu H., Kreuzer M. P., Takkinen K., Guilbault G.G. (2006). “A recombinant Fab fragment-based electrochemical immunosensor for the determination of testosterone in bovine urine” Biosensor and Bioelectronics. doi:10.1016/j.bios.2006.08.002. Cerca con Google

51. Lubet R.A., Mayer T.R., Cameroon J.W., Burke M.D., Nims R.W., Wolff T., Guengerich F.P. (1985) “Dealkylation of pentoxresorufme: a rapid and sensitive assay for measuring induction of cythocrome P450 by phenobarbital and other xenobiotics in the rats” Archives of Biochemhtry and Biophysic, 283, 43-48. Cerca con Google

52. Luo G., Guenthner T., Gan L.S., Humphreys W.G. (2004) “CYP3A4 induction by xenobiotics: biochemistry, experimental methods and impact on drug discovery and development” Curr Drug Metab., 5, 483-505 Cerca con Google

53. Meyer H.H. (2001) “Biochemistry and physiology of anabolic hormones used for improvement of meat production” APMIS, 109, 1-8. Cerca con Google

54. Merlanti R., Ferranti C., Ferretti G., De Liquoro M., Civitareale C., Gallina G., Draisci R. & Montesissa C. (2006). “Oral administration of boldenone and boldione to veal calves: disposition and elimination rate of boldenone metabolites”. J. vet. Pharmacol. Therap., 29 (suppl. 1), 167. Cerca con Google

55. Merlanti, R.; Gallina, G.; Capolongo, F.; Contiero, L.; Biancotto, G.; Dacasto M.; Montesissa, C. (2007) An in vitro study on metabolism of 17?-boldenone and boldione using cattle liver and kidney subcellular fractions. Anal. Chim. Acta , 586, 177-183. Cerca con Google

56. Nebbia C., Dacasto M.,. Rossetto Giaccherino A.,Giuliano Albo A. et Carletti M. (2003) “Comparative expression of liver cytochrome P450-dependent monooxygenases in the horse and in other agricultural and laboratory species” The Veterinary Journal, 165, 53-64. Cerca con Google

57. Nebbia C. (2006) “Measuring biological effects in the target species using proteomics: detection of the illegal treatment of cattle with performance enhancing agents” J. vet. Pharmacol. Therap., 29 (suppl. 1), 37-39 Cerca con Google

58. Nebert D.W., Nelson D.R., Coon M.J., Estabrook R.W., Feyereisen R., Fujii-Kuriyama Y., Gonzalez F.J., Guenguerich F.P., Gunsalus I.C., Johnson EF., Loper J.C., Sato R., Waterman M.R., Waxman D. (1991) “ The P450 superfamily: Update on new sequences, genes mapping, and raccomended nomenclature “ DNA and Celi Biology, 10, 1-14 Cerca con Google

59. Nielen M.W.F., Rutgers P., van Bennekom E.O., Lasaroms J.J.P., van Rhijn J.A.H. (2004) “Confirmatory analysis of 17?-boldenone, 17?-boldenone and androsta-1,4-diene-3,17-dione in bovine urine, faeces, feed and skin swab samples by liquid chromatography.electrospray ionisation tandem mass spectrometry” Journal of chromatography B, 801, 273-283 Cerca con Google

60. Nielen, M.W.F.; Lasaroms, J.P.; Essers, M.L.; Sanders, M.B.; Heskamp, H.H.; Bovee, T.F.H.; Van Rhijn, J.; Groot, M.J. (2007) The ultimate veal calf reference experiment: hormone residue analysis data obtained by gas and liquid chromatography tandem mass spectrometry. Anal. Chim. Acta, 586, 30-34 Cerca con Google

61. Ohno M., Yamaguchi I., Ito T., Saiki K., Yamamoto I., Azuma J. (2000) “Circadian variation of the urinary 6beta-hydroxycortisol to cortisol ratio that would reflect hepatic CYP3A activity” Eur J Clin Pharmacol., 55, 861-865 Cerca con Google

62. Okey A.B., Roberts E.A., Harper P.A. et Denison M.S. (1986) “Induction of drug-metabolizing enzymes: mechanisms and consequences” Clin Biochem., 19, 132-41 Cerca con Google

63. Paine A. J. (1991) “The cytochrome P450 gene superfamily (current status review)” International Journal of Experimental Pathology, 72, 349-363 Cerca con Google

64. Pascussi J.M., Gerbal-Chaloin S., Drocourt L., Maurel P., Vilarem M.J. (2003) " The expression og CYP2B6, CYP2C9 dand CYP3A4 genes: a tangle of networks of nuclear and steroid receptors" Biochimica and Biophysica Acta, 1619, 243-253 Cerca con Google

65. Pierrat B., Heery D.M., Lemoine Y., Losson R. (1992) “Functional analysis of the human estrogen receptor using a phenotypic transactivation assay in yeast” Gene, 119,(2), 237-45 Cerca con Google

66. Poelmans, S.;, De Wasch, K.; Martelé, Y.; Schilt, R.; Van Hoof, N.; Noppe, H.; Verslycke, T.; Janssen, C.R.; Courtheyn D.; De Brabander, H.F. (2003 a) The possible transformation of phytosterols to boldenone. In Proceedings of the Euro Food Chem XII Brugge, Belgium, 74-78 Cerca con Google

67. Poelmans S., Schilt R., Stephany R.W., Le Bizec B., Draisci R., Sterk S., Van Ginkel L. A., Courtheyn D., Van Hoof N., Lucentini L., De Wasch K., De Brabander H. F. (2003 b) Outcome of the experts meeting on the control of Boldenone in veal calves. European Commission, Health and Consumer Protection Directorate General, Brussels, 2003, 1-3 Cerca con Google

68. Poelmans S., De Wasch K., Noppe H., Van Hoof N., Van Cruchten S., Le Bizec B., Deceuninck Y., Sterk S., Van Rossum H.J., Hoffman M.K., De Brabander H.F. (2005 a) “Endogenous occurrence of some anabolic steroids in swine matrices” Food Addit Contam., 22, 808-815 Cerca con Google

69. Poelmans S., De Wasch K., Noppe H., Van Hoof N., Van de Wiele M., Courtheyn D., Gillis W., Vanthemsche P., Janssen C.R., De Brabander H.F. (2005 b). “Androstadienetrione, a boldenone-like component, detected in cattle faeces with GC-MS(n) and LC-MS(n)”. Food Addit Contam. 22(9):798-807 Cerca con Google

70. Poli G. (2005) “Diagnostica sierologia le reazioni antigene-anticorpo in vitro” in Microbiologia e immunologia veterinaria - 2° Ed.” UTET Medica, Torino, 662-664 Cerca con Google

71. Pompa G., Arioli F., Fracchiolla M.L., Rossi C.A., Bassini A.L., Stella S., Biondi P.A. (2006). “Neoformation of boldenone and related steroids in faeces of veal calves”. Food Addit Contam. 23(2):126-32. Cerca con Google

72. Porter T.D., Coon M.J. (1991) “Cytochrome P450: multiplicity of isoforms, substrates, and catalytic and regulatory mechanisms” Journal of Biological Chemistry, 266, 13469-13472. Cerca con Google

73. Preston R.L. (1999). “Hormone containing growth promoting implants in farmed livestock”. Advanced Drug Delivery Reviews 38, 123-138 Cerca con Google

74. Prévost S., Nicol T., Monteau F., André F., Le Bizec B. (2001) “Gas chromatography/combustion/isotope ratio mass spectrometry to control the misuse of androgens in breeding animals: new derivatisation method applied to testosterone metabolites and precursors in urine samples” Rapid. Commun. Mass Spectrom., 15, 2509-2514 Cerca con Google

75. Purdon M.P. and Lehman-McKeeman L.D. (1997) “Improved hight-performance liquid chromatografic procedure for separation and quantification of hydroxytestosterone metabolites” Journal of Pharmacology and Toxycology, 37, 67-73. Cerca con Google

76. Reinerink E.J., Doorn L., Jansen E.H.J., Van Tersel A.A.J. (1991) “Measurement of enzyme activities of cytochrome P-450 isoenzymes by high-performance liquid chromatographic analyzis ofproducts”, Journal ofChromatography, 553, 233-241 Cerca con Google

77. Rico A.G. (1983) “Metabolism of endogenous and exogenous anabolic agents in cattle” J. Anim. Sci., 57, (1), 226-32 Cerca con Google

78. Rutten A.J.J.L., Falke H.E., Catsburg J.F., Top R., Blaauboer B.J., Van Holstein I., Doorn L., Van Leeuwen F.X.R. (1987) “Interlaboratory comparison of total cytochrome P450 and protein determinations in rat liver microsomes” Archives of Toxicology, 61, 27-33. Cerca con Google

79. Samuels T.P., Nedderman A., Seymour M.A. et E. Houghton (1998) “Study of the metabolism of testosterone, nandrolone and estradiol in cattle” Analyst, 123, 2401-2404. Cerca con Google

80. Sangiorgi, E.; Polignano, V.; Gardini, S. (2005) Boldenone and related hormones quantification by liquid chromatography’mass spectrometry in urine and faeces after calf administration of boldenone undecanoate. Anal. Chim. Acta , 529, 239-248 Cerca con Google

81. Saudan C., Baume N., Robinson N., Avois L., Mangin P., Saugy M. (2006) « Testosterone and doping control” Br J Sports Med., 40 (Suppl 1), 21-24 Cerca con Google

82. Sauer M.J., Samuels T.P., Howells L.G., Seymour M.A., Nedderman A., Houghton E., Bellworthy S.J., Anderson S., Coldham N.G. (1998) “Residues and metabolism of 19-nortestosterone laurate in steers” Analyst, 123, 2653-2660 Cerca con Google

83. Schanzer, W., Donike, M. (1992) “Metabolism of boldenone in man: gas chromatographic/mass spectrometric identification of urinary excreted metabolites and determination of excretion rates” Biol. Mass. Spectrom., 21, 3-16. Cerca con Google

84. Schänzer W. (1996) “Metabolism of anabolic androgenic steroids” Clin. Chem,. 42, 1001-1020 Cerca con Google

85. Schimmer B.P, Parker K.L. (2006) “Adrenocorticotropic hormone; adrenocortical steroids and their synthetic analogs; inhibitors of the synthesis and actions of adrenocortical hormones” in “Goodman & Gilman's the Pharmacological Basis of Therapeutics 11th Ed.” (editori: Brunton L., Lazo J., Parker K.L.) McGraw Hill, New York Cerca con Google

86. Scippo M., Gaspar P., Degand G., Maghuin-Rogister G. (1993) “Control of the illegal administration of natural steroid hormones in urine and tissues of veal calves and in plasma of bulls” Analytica Chimica Acta, 275; 57-74. Cerca con Google

87. Scippo M., Degand G., Duyckaerts A. et G. Maghuin-Rogister (1994) “Control of the illegal administration of natural steroid hormones in the plasma of bulls and heifers” Analyst, 119, 2639-2644. Cerca con Google

88. Scippo M.L., van de Weerdt, C., Willemsen, P., Francois J. M., Rentier-Delrue F., Muller M., Martial J. A., Maghuin Rogister G. (2002). “Detection of illegal growth promoters in biological samples using receptor binding assays” Analytica-Chimica-Acta. 473(1/2): 135-141. Cerca con Google

89. Serratosa J., Blass A., Rigau B., Mongrell B., Rigau T., Tortadès M., Tolosa E., Aguilar C., Ribó O., Balagué J. (2006) “Residues from veterinary medicinal products, growth promoters and performance enhancers in food-producing animals: a European Union perspective” Rev. Sci. Tech., 25, 637-53 Cerca con Google

90. Sevaljevi? L., Isenovi? E., Vulovi? M., Macvanin M., Zakula Z., Kanazir D., Ribarac-Stepi? N. (2001) “The responses of rat liver glucocorticoid receptors and genes for tyrosine aminotransferase, alpha-2-macroglobulin and gamma-fibrinogen to adrenalectomy-, dexamethasone- and inflammation-induced changes in the levels of glucocorticoids and proinflammatory cytokines” Biol. Signals Recept., 10, 299-309 Cerca con Google

91. Sgoifo Rossi C.A., Arioli F., Bassini A., Chiesa L.M., Dell’Orto V., Montana M., Pompa G. (2004) “Evidence for false-positive results for boldenone testing of veal calves due to faecal cross-contamination during sampling” Food Addit Contam, 21 (8), 756-762 (7). Cerca con Google

92. Silvan G., Martínez-Mateos M.M., Blass A., Camacho L., Gonzalez-Gil A., Garcia-Partida P., Illera J.C. (2007) “The effect of long-term exposure to combinations of growth promoters in Long Evans rats: part 1: Endocrine adrenal function” Anal Chim Acta, 586, 246-251 Cerca con Google

93. Simontacchi C., Bono G., Bongioni G., Gabai G., Ferasin L., Borghese A. (1997) “Manuale di pratiche di laboratorio per la validazione di kit commerciali per il dosaggio degli ormoni steroidei in campo animale” Zoot. Nutr. Anim., 23, 281-288 Cerca con Google

94. Simontacchi C., Marinelli L., Gabai G., Bono G. et R. Angeletti (1999) “Accuracy in naturally occuring anabolic steroid assays in cattle and first approach to quality control in Italy” Analyst, 124, 307-312. Cerca con Google

95. Simontacchi C, Perez de Altamirano T, Marinelli L, Angeletti R, Gabai G. (2004). “Plasma steroid variations in bull calves repeatedly treated with testosterone, nortestosterone and oestradiol administered alone or in combination”. Vet Res Commun. 28(6):467-77. Cerca con Google

96. Sivapathasundaram S., Magnisali P., Coldham N.G., Howells L.C., Sauer M. J. et Ioannides C. (2001) “A study of the expression of the xenobiotic metabolising cytochrome P450 proteins and of testosterone metabolism in bovine liver” Biochemical Pharmacology, 62, 635-45. Cerca con Google

97. Snell K., Mullock B. (1987) “Biochemical Toxicology: A pratical approach” IRL, Oxford University Press, Oxford, England. Cerca con Google

98. Song Y.S., Jin C., Park E.H. (2000) “Identification of metabolites of phytosterols in rat feces using GC/MS.” Arch. Phar. Res., 23, (6), 599-604. Cerca con Google

99. Starka L. (2003) “Epitestosterone” Journal of Steroid Biochemistry & Molecular Biology, 87, 27-34. Cerca con Google

100. Sterk, S.S.; Blokland, M.H.; Van Ginkel, L.A.;R.Schilt, Van der Vlis, E.; Boshuis, P.; Van Baak, M.J.; Nielen, M.W.F.; Van Rhijn, J.A.; Samson, D. ; Keukens, H.J.; Stephany, R.W.(2004) “Boldenone: an overview of Dutch research carried out in the veterinary field” In Proceedings of the EuroResidue V; Van Ginkel, L.A., Bergwerff A.A., Eds.; Nordwijkerhout The Netherlands, 900-906. Cerca con Google

101. Szotáková B., Baliharová V., Lamka J., Nožinová E., Wsól V., Velík J., Machala M., Ne?a J., Sou?ek P., Šusová S., Skálová L. (2004) “Comparison of in vitro activities of biotransformation enzyme in pig, cattle, goat and sheep” Research of Veterinary Science, 76, 43-51 Cerca con Google

102. Touber M.E., van Engelen M.C., Georgakopoulus C., van Rhijn J.A., Nielen M.W. (2007) “Multi-detection of corticosteroids in sports doping and veterinary control using high-resolution liquid chromatography/time-of-flight mass spectrometry” Anal Chim Acta, 586, 137-146 Cerca con Google

103. Totsuka S., Watanabe T., Koyanagi F., Tanaka K., Yasuda M., Manabe S.(1999) “Increase in urinary excretion of 6beta-hydroxycortisol in common marmosets as a marker of hepatic CYP3A induction” Arch Toxicol.,73, 203-207 Cerca con Google

104. Van den Hauwe O., Campbell K., Crooks S.R., Schilt R., Van Peteghem C.H. (2005 a) “Confirmation of synthetic glucocorticoids with liquid chromatography/mass spectrometry: organization and results of an international interlaboratory comparison test” J AOAC Int., 88, 87-94 Cerca con Google

105. Van den Hauwe O., Dumoulin F., Elliott C., Van Peteghem C. (2005 b) “Detection of synthetic glucocorticoid residues in cattle tissue and hair samples after a single dose administration using LC-MS/MS” J Chromatogr B Analyt Technol Biomed Life Sci.,817, 215-223 Cerca con Google

106. Van de Kerkhof D.H, de Boer D., Thijssen J.H., Maes R.A. (2000) “Evaluation of testosterone/epitestosterone ratio influential factors as determined in doping analysis” J. Anal. Toxicol. 24, 102-115 Cerca con Google

107. Van Peteghem C., Van Poucke C. (2006) “Analytical Methods: what is feasible nowadays? The state of the art” J. vet. Pharmacol. Therap., 29 (suppl. 1), 33-34 Cerca con Google

108. Van Puymbroeck M., Kuilman M.E.M., Maas R.F.M.., Witkamp R.F, Leyssens L., Vanderzande D., Gelan J., Raus J. (1998 a) “In vitro models are important tools to monitor the abuse of anabolic steroids in cattle” Analyst, 123, 2453-2456. Cerca con Google

109. Van Puymbroeck M., Kuilman M.E.M., Maas R.F.M., Witkamp R.F, Leyssens L., Vanderzande D., Gelan J., Raus J. (1998 b) “Identification of some important metabolites of boldenone in urine and feces of cattle by gas chromatography-mass spectrometry” Analyst, 123, 2681-2686. Cerca con Google

110. Van Puymbroeck M., Kuilman M.E., Maas R.F., Witkamp R.F., Leyssens L., Van Miert A.S., Hendriks L., Vanderzande D., Adriaensens P., Jacobs M.P., Raus J. (1999) “17alpha-ethyl-5beta-estrane-3alpha, 17beta-diol, a biological marker for the abuse of norethandrolone and ethylestrenol in slaughter cattle” J Chromatogr B Biomed Sci Appl., 728, 217-232 Cerca con Google

111. Van’t Klooster G.A.E. (1992 a) “Drug metabolism in ruminants an in vitro approach” (Tesi di dottorato), Wageningen. Cerca con Google

112. Van’t Klooster G.A.E., Blaauboer B.J., Noordhoek J., Van Miert A.S.J.P.A.M. (1992 b) “Cytochrome P450 induction and metabolism of alkoxyresorufins, ethylmorphine and testosterone in isolated hepatocytes from goats, sheep and cattle” Biochemical Pharmacology, 46, 1781-90. Cerca con Google

113. Wolfe R., Ferrando A., Sheffield-Moore M. et R. Urban (2000) “Testosterone and muscle protein metabolism” Mayo. Clin. Proc., 75, 55-59. Cerca con Google

114. Wu F.C. (1997) “Endocrine aspects of anabolic steroid” Clin. Chem., 43, 1289-1292. Cerca con Google

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