Favaretto, Francesca (2008) ObesitĂ , insulino resistenza e nuove molecole segnale: studi nell'uomo e in modelli animali. [Ph.D. thesis]
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In this work we realized a molecular and functional characterization of P2 receptors in several adipose tissue depots (subcuteneous, visceral, preadipocytes/stromal vascular fraction and adipocytes by RT-PCR, qPCR, sequencing, fluorescent dye Ca2+ sensitive, and immunoenzymatic assay. We evaluated also a gene expression in dermal fibroblasts from Alstrom patients using DNA microarray analysis. We found a modulation of genes coding proteins cell cycle related, extracellular matrix, cellular movement and insulin pathway.
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