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Dal Molin, Federica - Tonello , Fiorella - Ladant, Daniel - Zornetta, Irene - Zamparo, Ilaria - Di Benedetto , Giulietta - Zaccolo , Manuela - Montecucco , Cesare (2006) Cell entry and cAMP imaging of anthrax edema toxin. [Articolo di periodico (online)]

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Abstract (inglese)

The entry and enzymatic activity of the anthrax edema factor (EF) in different cell types was studied by monitoring EF-induced changes in intracellular cAMP with biochemical and microscopic methods. cAMP was imaged in live cells, transfected with a fluorescence resonance energy transfer biosensor based on the protein kinase A regulatory and catalytic subunits fused to CFP and YFP, respectively. The cAMP biosensor was located either in the cytosol or was membrane-bound owing to the addition of a tag determining its myristoylation/palmitoylation. Real-time imaging of cells expressing the cAMP biosensors provided the time course of EF catalytic activity and an indication of its subcellular localization. Bafilomycin A1, an inhibitor of the vacuolar ATPase proton pump, completely prevented EF activity, even when added long after the toxin. The time course of ppearance of the adenylate cyclase activity and of bafilomycin A1 action suggests that EF enters the cytosol from late endosomes. EF remains associated to these compartments and its activity shows a perinuclear localization generating intracellular cAMP concentration gradients from the cell centre to the periphery.


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Tipo di EPrint:Articolo di periodico (online)
Anno di Pubblicazione:02 Novembre 2006
Parole chiave (italiano / inglese):anthrax, cAMP, cell entry, edema toxin, FRET imaging
Settori scientifico-disciplinari MIUR:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Struttura di riferimento:Dipartimenti > Dipartimento di Scienze Biomediche Sperimentali
Codice ID:984
Depositato il:24 Feb 2008
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Abrami L, Liu S, Cosson P, Leppla SH, Van der Goot FG (2003) Anthrax toxin triggers endocytosis of its receptor via a lipid raft-mediated clathrin-dependent process. J Cell Biol 160: 321—328 [PubMed]. Cerca con Google

Abrami L, Lindsay M, Parton RG, Leppla SH, Van der Goot FG (2004) Membrane insertion of anthrax protective antigen and cytoplasmic delivery of lethal factor occur at different stages of the endocytic pathway. J Cell Biol 166: 645—651 [PubMed]. Cerca con Google

Abrami L, Reig N, Van der Goot FG (2005) Anthrax toxin: the long and winding road that leads to the kill. Trends Microbiol 13: 72—78 [PubMed]. Cerca con Google

Agrawal A, Lingappa J, Leppla SH, Agrawal S, Jabbar A, Quinn C, Pulendran B (2003) Impairment of dendritic cells and adaptive immunity by anthrax lethal toxin. Nature 424: 329—334 [PubMed]. Cerca con Google

Ascenzi P, Visca P, Ippolito G, Spallarossa A, Bolognesi M, Montecucco C (2002) Anthrax toxin: a tripartite lethal combination. FEBS Lett 531: 384—388 [PubMed]. Cerca con Google

Baldari T, Tonello F, Rossi-Paccani S, Montecucco C (2006) Anthrax toxins: a paradigm of bacterial immune suppression. Trends Immunol 27: 434—440 [PubMed]. Cerca con Google

Beauregard KE, Wimer-Mackin S, Collier RJ, Lencer WI (1999) Anthrax toxin entry into polarized epithelial cells. Infect Immun 67: 3026—3030 [PubMed]. Cerca con Google

Bradley KA, Mogridge J, Mourez M, Collier RJ, Young JA (2001) Identification of the cellular receptor for anthrax toxin. Nature 414: 225—229 [PubMed]. Cerca con Google

Collier RJ, Young JA (2003) Anthrax toxin. Annu Rev Cell Dev Biol 19: 45—70 [PubMed]. Cerca con Google

Dixon TC, Meselson M, Guillemin J, Hanna PC (1999) Anthrax. N Engl J Med 341: 815—826 [PubMed]. Cerca con Google

Dong C, Davis RJ, Flavell RA (2002) MAP kinases in the immune response. Annu Rev Immunol 20: 55—72 [PubMed]. Cerca con Google

Drum CL, Yan SZ, Sarac R, Mabuchi Y, Beckingham K, Bohm A, Grabarek Z, Tang WJ (2000) An extended conformation of calmodulin induces interactions between the structural domains of adenylyl cyclase from Bacillus anthracis to promote catalysis. J Biol Chem 275: 36334—36340 [PubMed]. Cerca con Google

Duesbery NS, Webb CP, Leppla SH, Gordon VM, Klimpel KR, Copeland TD, Ahn NG, Oskarsson MK, Fukasawa K, Paull KD, Vande Woude GF (1998) Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science 280: 734—737 [PubMed]. Cerca con Google

Evellin S, Mongillo M, Terrin A, Lissandron V, Zaccolo M (2004) Measuring dynamic changes in cAMP using fluorescence resonance energy transfer. Methods Mol Biol 284: 259—270 [PubMed]. Cerca con Google

Firoved AM, Miller GF, Moayeri M, Kakkar R, Shen Y, Wiggins JF, McNally EM, Tang WJ, Leppla SH (2005) Bacillus anthracis edema toxin causes extensive tissue lesions and rapid lethality in mice. Am J Pathol 167: 1309—1320 [PubMed]. Cerca con Google

Friedlander AM (1986) Macrophages are sensitive to anthrax lethal toxin through an acid-dependent process. J Biol Chem 261: 7123—7126 [PubMed]. Cerca con Google

Gaestel M (2006) MAPKAP kinases—MKs—two's company, three's a crowd. Nat Rev Mol Cell Biol 7: 120—130 [PubMed]. Cerca con Google

Gordon VM, Young WW Jr, Lechler SM, Gray MC, Leppla SH, Hewlett EL (1989) Adenylate cyclase toxins from Bacillus anthracis and Bordetella pertussis. Different processes for interaction with and entry into target cells. J Biol Chem 264: 14792—14796 [PubMed]. Cerca con Google

Gruenberg J (2001) The endocytic pathway: a mosaic of domains. Nat Rev Mol Cell 2: 721—730. Cerca con Google

Gruenberg J, Griffiths G, Howell KE (1989) Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in vitro. J Cell Biol 108: 1301—1316 [PubMed]. Cerca con Google

Gruenberg J, van der Goot G (2006) Mechanism of pathogen entry thorugh the endosomal compartments. Nat Rev Mol Cell Biol 7: 495—504 [PubMed]. Cerca con Google

Guidi-Rontani C, Weber-Levy M, Mock M, Cabiaux V (2000) Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes. Cell Microbiol 2: 259—264 [PubMed]. Cerca con Google

Hewlett EL, Kim KJ, Lee SJ, Gray MC (2000) Adenylate cyclase toxin from Bordetella pertussis: current concepts and problems in the study of toxin functions. Int J Med Microbiol 290: 333—335 [PubMed]. Cerca con Google

Karimova G, Fayolle C, Gmira S, Ullmann A, Leclerc C, Ladant D (1998) Charge-dependent translocation of Bordetella pertussis adenylate cyclase toxin into eukaryotic cells: implication for the in vivo delivery of CD8+ T-cell epitopes into antigen-presenting cells. Proc Natl Acad Sci USA 95: 12532—12537 [PubMed]. Cerca con Google

Kobayashi T, Beuchat MH, Chevallier J, Makino A, Mayran N, Escola JM, Lebrand C, Cosson P, Kobayashi T, Gruenberg J (2002) Separation and characterization of late endosomal membrane domains. J Biol Chem 277: 32157—32164 [PubMed]. Cerca con Google

Kochi SK, Martin I, Schiavo G, Mock M, Cabiaux V (1994) The effects of pH on the interaction of anthrax toxin lethal and edema factors with phospholipid vesicles. Biochemistry 33: 2604—2609 [PubMed]. Cerca con Google

Krantz BA, Melnyk RA, Zhang S, Juris SJ, Lacy DB, Wu Z, Finkelstein A, Collier RJ (2005) A phenylalanine clamp catalyzes protein translocation through the anthrax toxin pore. Science 309: 777—781 [PubMed]. Cerca con Google

Ladant D, Ullmann A (1999) Boreatella pertussis adenylate cyclase: a toxin with multiple talents. Trends Microbiol 7: 172—176 [PubMed]. Cerca con Google

Lissandron V, Terrin A, Collini M, D'Alfonso L, Chirico G, Pantano S, Zaccolo M (2005) Improvement of a FRET-based indicator for cAMP by linker design and stabilization of donor—acceptor interaction. J Mol Biol 354: 546—555 [PubMed]. Cerca con Google

Menard A, Altendorf K, Breves D, Mock M, Montecucco C (1996) The vacuolar ATPase proton pump is required for the cytotoxicity of Bacillus anthracis ethal toxin. FEBS Lett 386: 161—164 [PubMed]. Cerca con Google

Moayeri M, Leppla SH (2004) The roles of anthrax toxin in pathogenesis. Curr Opin Microbiol 7: 19—24 [PubMed]. Cerca con Google

Mock M, Mignot T (2003) Anthrax toxins and the host: a story of intimacy. Cell Microbiol 5: 15—23 [PubMed]. Cerca con Google

Mongillo M, McSorley T, Evellin S, Sood A, Lissandron V, Terrin A, Huston E, Hannawacker A, Lohse MJ, Pozzan T, Houslay MD, Zaccolo M (2004) Fluorescence resonance energy transfer-based analysis of cAMP dynamics in live neonatal rat cardiac myocytes reveals distinct functions of compartmentalized phosphodiesterases. Circ Res 95: 67—75 [PubMed]. Cerca con Google

Mongillo M, Terrin A, Evellin S, Lissandron V, Zaccolo M (2005) Study of cyclic adenosine monophosphate microdomains in cells. Methods Mol Biol 307: 1—13 [PubMed]. Cerca con Google

Paccani SR, Tonello F, Ghittoni R, Natale M, Muraro L, D'Elios MM, Tang WJ, Montecucco C, Baldari CT (2005) Anthrax toxins suppress T lymphocyte activation by disrupting antigen receptor signaling. J Exp Med 201: 325—331 [PubMed]. Cerca con Google

Papini E, Rappuoli R, Murgia M, Montecucco C (1993) Cell penetration of diphtheria toxin. Reduction of the interchain disulfide bridge is the rate-limiting step of translocation in the cytosol. J Biol Chem 268: 1567—1574 [PubMed]. Cerca con Google

Park JM, Greten FR, Li ZW, Karin M (2002) Macrophage apoptosis by anthrax lethal factor through p38 MAP kinase inhibition. Science 297: 2048—2051 [PubMed]. Cerca con Google

Pellizzari R, Guidi-Rontani C, Vitale G, Mock M, Montecucco C (1999) Anthrax lethal factor cleaves MKK3 in macrophages and inhibits the LPS/IFNgamma-induced release of NO and TNFalpha. FEBS Lett 462: 199—204 [PubMed]. Cerca con Google

Rainey GJ, Wigelsworth DJ, Ryan PL, Scobie HM, Collier RJ, Young JA (2005) Receptor-specific requirements for anthrax toxin delivery into cells. Proc Natl Acad Sci USA 102: 13278—13283 [PubMed]. Cerca con Google

Scobie HM, Rainey GJ, Bradley KA, Young JA (2003) Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc Natl Acad Sci USA 100: 5170—5174 [PubMed]. Cerca con Google

Shen Y, Zhukovskaya NL, Guo Q, Florian J, Tang WJ (2005) Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor. EMBO J 24: 929—941 [PubMed]. Cerca con Google

Simpson IA, Sonne O (1982) A simple, rapid and sensitive method for measuring protein concentration in subcelluler membrane fractions prepared by sucrose density ultracentrifugation. Anal Biochem 119: 424—427 [PubMed]. Cerca con Google

Tournier JN, Quesnel-Hellmann A, Mathieu J, Montecucco C, Tang WJ, Mock M, Vidal DR, Goossens PL (2005) Anthrax edema toxin cooperates with lethal toxin to impair cytokine secretion during infection of dendritic cells. J Immunol 174: 4934—4941 [PubMed]. Cerca con Google

Turnbull PC (2002) Introduction: anthrax history, disease and ecology. Curr Top Microbiol Immunol 271: 1—19 [PubMed]. Cerca con Google

Vitale G, Bernardi L, Napolitani G, Mock M, Montecucco C (2000) Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor. Biochem J 352: (Part 3): 739—745 [PubMed]. Cerca con Google

Vitale G, Pellizzari R, Recchi C, Napolitani G, Mock M, Montecucco C (1998) Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine/threonine phosphorylation of MAPKs in cultured macrophages. Biochem Biophys Res Commun 248: 706—711 [PubMed]. Cerca con Google

Wei W, Lu Q, Chaudry GJ, Leppla SH, Cohen SN (2006) The LDL receptor-related protein LRP6 mediates internalization and lethality of anthrax toxin. Cell 124: 1119—1121 [PubMed]. Cerca con Google

Wigelsworth DJ, Krantz BA, Christensen KA, Lacy DB, Juris SJ, Collier RJ (2004) Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. J Biol Chem 279: 23349—23356 [PubMed]. Cerca con Google

Zaccolo M, De Giorgi F, Cho CY, Feng L, Knapp T, Negulescu PA, Taylor SS, Tsien RY, Pozzan T (2000) A genetically encoded, fluorescent indicator for cyclic AMP in living cells. Nat Cell Biol 2: 25—29 [PubMed]. Cerca con Google

Zacharias DA, Violin JD, Newton AC, Tsien RY (2002) Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296: 913—916 [PubMed]. Cerca con Google

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