Vai ai contenuti. | Spostati sulla navigazione | Spostati sulla ricerca | Vai al menu | Contatti | Accessibilità

| Crea un account

Collavo, Alberto - Scarabel, Laura - Campagna, Claudio - Sattin, Maurizio (2007) Characterisation of ACCase inhibitors resistant Phalaris paradoxa. In: 14th EWRS Symposium, 17-21 June 2007, Hamar, Norway.

Full text disponibile come:

Documento PDF

Abstract (inglese)

Phalaris paradoxa L. (Poaceae family) - hood canarygrass - is an annual grass infesting winter cereals in central and southern Italy. This study investigated the resistance status of seven Italian populations of P. paradoxa collected from fields where an inadequate level of control was observed following an extensive history of ACCase applications. The aims were also to determine the efficacy of alternative herbicides and investigate the molecular basis of one highly resistant population. Two outdoor pot dose-response experiments were done to collect information on patterns and levels of cross-resistance. Seven-eight herbicide doses (plus an untreated check) were used for each of the following herbicides: clodinafop-propargyl (240 g/L), diclofop-methyl (284 g/L), fenoxaprop (55 g/L), sethoxydim 20% (185 g/L), tralkoxydim 22.5% (250 g/L), pinoxaden (100 g/L), chlorsulfuron (75 g/L), iodosulfuron (50 g/Kg), imazamethabenz (300 g/L) and isoproturon (500 g/L). Each experiment had three replicates with three pots per replicate containing 6 plants each and also included a susceptible population. The experimental design was completely randomised. Biomass and plant survival data were collected 18-21 days after herbicides application. The mean survival and fresh weight for each treatment were expressed as a percentage of the untreated control treatments. The ED50, GR50, for the mean percentage survival and fresh weight respectively, were calculated using non linear regression analysis. Statistical analysis was conducted using an EXCEL’®VBA macro based on a log-logistic equation to fit the data. Resistance indexes (R.I.) were calculated from ED50 and GR50 values (GR50R/GR50S, ED50R/ED50S). Two populations showed a low level of resistance to diclofop (fresh weight R.I. of 2.5 and 3.4), while only one population displayed a wide pattern of cross-resistance to ACCase herbicides, including sethoxydim (R.I. 40.5) and pinoxaden (R.I. 15.1). This suggested that the resistance mechanism is target site mediated. Genomic PCR amplification and direct sequencing of the carboxyl transferase domain of the ACCase gene pinpointed the presence of an Ile1781 to Val1781 substitution and a Asp2078 to Val2078 in the P. paradoxa chloroplastic ACCase of the resistant population. These mutations has already been shown to confer resistance to ACCase inhibitors in other grass weeds, but these results document the first case of an ACCase-resistant population of P. paradoxa in Europe. Non-ACCase-inhibiting herbicides with mode of action belonging to the PSII (isoproturon) and ALS inhibitors (iodosulfuron, chlorsulfuron, imazamethabenz) adequately controlled all the tested P. paradoxa populations.

Statistiche Download - Aggiungi a RefWorks
Tipo di EPrint:Contributo a convegno (Presentazione)
Anno di Pubblicazione:Giugno 2007
Parole chiave (italiano / inglese):phalaris paradoxa ACCase inhibitors target site resistance 1781 2078 pinoxaden diclofop
Settori scientifico-disciplinari MIUR:Area 07 - Scienze agrarie e veterinarie > AGR/02 Agronomia e coltivazioni erbacee
Struttura di riferimento:Dipartimenti > Dipartimento di Agronomia Ambientale e Produzioni Vegetali
Codice ID:988
Depositato il:05 Feb 2008
Simple Metadata
Full Metadata
EndNote Format


I riferimenti della bibliografia possono essere cercati con Cerca la citazione di AIRE, copiando il titolo dell'articolo (o del libro) e la rivista (se presente) nei campi appositi di "Cerca la Citazione di AIRE".
Le url contenute in alcuni riferimenti sono raggiungibili cliccando sul link alla fine della citazione (Vai!) e tramite Google (Ricerca con Google). Il risultato dipende dalla formattazione della citazione.

Baldini, R.M., 1993. The genus Phalaris L. (Gramineae) in Italy. Webbia 47 (1):1-53. Cerca con Google

Bourgeois,-L; Kenkel,-N-C; Morrison,-I-N, 1997. Characterization of cross-resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua). Weed-Science. 1997; 45(6): 750-755 Cerca con Google

Bravin F, Zanin G & Preston C (2001) Diclofop-methyl resistance in populations of Lolium spp. from central Italy. Weed Research 41, 49-58. Cerca con Google

Burnet,-M-W-M; Hart,-Q; Holtum,-J-A-M; Powles,-S-B, 1994. Resistance to nine herbicide classes in a population of rigid ryegrass (Lolium rigidum). Weed-Science 42(3): 369-377. Cerca con Google

Heap, I.M., 2005. International survey of herbicides-resistant weeds, Weed Science Society of America and the Herbicide Action Committee. Online, Internet 16 maggio 2005, Vai! Cerca con Google

Liu WJ, Harrison DK, Chalupska D, Gornicki P, O’donnell CC, Adkins SW, Haselkorn R, Williams RR (2007) Single-site mutations in the carboxyltransferase domain of plastid acetyl-CoA carboxylase confer resistance to grass-specific herbicides. Proc Natl Acad Sci USA 104: 3627 - 3632 Cerca con Google

Lucchesi, G. and Sattin M., 2002. Characterisation of ACCase herbicide resistant Phalaris paradoxa in Italy. In: Proc. XII EWRS Symposyum,2002, Papendal, The Netherlands. (in press) Cerca con Google

Konishi, T. and Y. Sasaki, 1994. Compartimentalization of two forms of Acetyl-CoA carboxylase in plants and the origin of their tolerance toward herbicides. Proc. Natl. Acad. Sci. USA Plant Biology 91: 3598-3601. Cerca con Google

Malik, R. K., H. Brown (ed.), G. W. Cussans (ed.), M. D. Devine (ed.), S. O. Duke (ed.), C. Fernandez Quintanilla (ed.), A. Helweg (ed.), R. E. Labrada (ed.), M. Landes (ed.), P. Kudsk (ed.), and J. C. Streibig. 1996. Herbicide resistant weed problems in developing world and methods to overcome them. Proceedings of the second international weed control congress, Copenhagen, Denmark, 25-28 June 1996. 1-4: 665-673. Cerca con Google

Menendez,-J; Prado,-R-de, 1996. Diclofop-methyl cross-resistance in a chlorotoluron-resistant biotype of Alopecurus myosuroides. Pesticide-Biochemistry-and-Physiology 56(2): 123-133. Cerca con Google

Onofri A., 2004. Bioassay97: EXCEL Add-in per l'elaborazione statistica del dosaggio biologico con erbicidi. In: Atti delle III Giornate di Studio su Metodi numerici, statistici e informatici nella difesa delle colture agrarie e delle foreste: ricerca ed applicazioni (A. Dalla Marta e S. Orlandini, Eds). Firenze, 24-26 Novembre 2004, 202-206. Cerca con Google

Porceddu E, Sattin M & Zanin G (1997) Herbicide Resistance in Italy: Evolution and Current situation. Agricoltura Mediterranea. 127, 97-105. Cerca con Google

Powels, S.B., and D.L. Shaner, 2001. Herbicides resistance and world grains, CRC Press LLC, Boca Raton, FL, 308 pp. Cerca con Google

Prieto-Baena, J.C, P.J. Hidalgo, E. Dominguez and C. Galan, 2003. Pollen production in the Poaceae family. Grana, 42: 153-160. Cerca con Google

Sattin M., Gasparetto M.A., Campagna C. 2001. Situation and management of Avena sterilis ssp ludoviciana and Phalaris paradoxa resistant to ACCase inhibitors in Italy. Proc. British Crop Protection Conference - Weeds, Brighton. : 755-562. Cerca con Google

Seefeldt S, Jensen JE & Fuerst EP (1995) Log-logistic analysis of herbicide dose-response relationships. Weed Technology 9, 218-227. Cerca con Google

Singh, B.K. and D.L. Shaner, 1995. Biosynthesis of branched chain amino acids: from test tube to field, Plant Cell, 7:935- Cerca con Google

Thill, D.C. and D. Lemerle, 2001. World wheat and resistance, in Herbicides resistance and world grains, CRC Press LLC, Boca Raton, FL, 308 pp. Cerca con Google

Yaacoby T, Schonfeld M & Rubin B (1985) Triazine-resistant grass weeds and their reponse to herbicides. Phytoparasitica 13, 232. Cerca con Google

Zanin G and Lucchin M (1996) Resistance to Acetyl-Coenzyme A Carboxylase inhibiting herbicides: Preliminary results on the resistance to diclofop-methyl of various Avena Sterilis biotypes in Italy. Agricoltura Mediterranea 126, 86-104. Cerca con Google

Download statistics

Solo per lo Staff dell Archivio: Modifica questo record